LOCUS       40924_480        8492 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Candida integrating vector CIp30, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Dennison PM, Ramsdale M, Manson CL, Brown AJ.
  TITLE     Gene disruption in Candida albicans using a synthetic, 
            codon-optimised Cre-loxP system
  JOURNAL   Fungal Genet. Biol. 42 (9), 737-748 (2005)
  PUBMED    16043373
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Dennison PMJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS, 
            Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE   3  (bases 1 to 8492)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2005"; volume: "42"; issue: "9"; pages: 
            "737-748"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2005) Medical Sciences, Aberdeen University, IMS, 
            Foresterhill, Aberdeen AB25 2ZD, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label=URA3
                     /note="Orotidine 5'-phosphate decarboxylase from Candida 
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#: 
                     P13649"
     misc_feature    2072..4076
                     /label=ARG4 marker
                     /note="ARG4 marker"
     CDS             complement(4131..5024)
                     /gene="HIS1"
                     /label=HIS1
                     /note="ATP phosphoribosyltransferase from Candida albicans 
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     misc_feature    5372..6232
                     /label=RPS1
                     /note="RPS1"
     primer_bind     complement(6258..6274)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6304..6322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6343..6359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6367..6383)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6391..6421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6436..6457)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6745..7333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7507..8364)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8365..8469)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcggcc gacttggcca agcctagatc ccgactaata ggaattgatt
      721 tggatggtat aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta
      781 ttatttgatt ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag
      841 ttgctgtagt gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat
      901 agtatccaaa aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac
      961 ataatgaaag aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa
     1021 acattgatca attcctgagc aacaacccca tacacactgg tttatatacc gcccctttta
     1081 cagttgaaga aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa
     1141 gacctatagt gagagagcag aaactcatgc ctcaccagta gcacagcgat tatttcgatt
     1201 aatggaactg aagaaaacca atttatgtgc atcaattgac gttgatacca ctaaggaatt
     1261 ccttgaatta attgataaat taggtcctta tgtatgctta atcaagactc atattgatat
     1321 aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca
     1381 tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg taaagaaaca
     1441 atatattggt ggagtttata aaattagtag ttgggcagat attaccaatg ctcatggtgt
     1501 cactgggaat ggagtggttg aaggattaaa acagggagct aaagaaacca ccaccaacca
     1561 agagccaaga gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg
     1621 agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt
     1681 tattgcccaa cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc
     1741 tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga
     1801 agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag
     1861 agatccagat attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa
     1921 gactggccaa ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt
     1981 agaataaata aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt
     2041 tacaatcaaa ggtggtcctt ctagaactag cttgatgctc gagggggatc cgtacccccc
     2101 tttagtaaga tttttcaaga gtagtctcaa ataaacagaa tgaaggattg aaggttttat
     2161 tcaataaata attggacaga aagttattgc acagttttaa caacaacaat ttcaaaattt
     2221 caatttatga catattgacc gacataattc agtcatttct aatattcaaa taacaagaca
     2281 ccttgtataa atgaatccac aagcgtatat gaacaaccaa attttatgac taactgtggg
     2341 tcgtgtactt ttgacgattg agtcattttt accaattagg taattgaatt ttactaattt
     2401 gtcattaacc gcgctggcca tcccaacaac aacaacaaca atcaatttaa aaacttgaca
     2461 aggcaaaaaa aaaaaatttc atctttgaaa aatttttctt ccttttcttc ttctgttctt
     2521 tacataacag ctatcaagaa taatcaatta ataatgtcac aacaacaaga taaacaacct
     2581 agtgaaaata aattatgggg tggtcgtttc accggtgcca ctgatccatt gatggatttg
     2641 tataatgctt cattacctta tgataaagtt atgtatgatg ctgatttaac tggtactaaa
     2701 gtatacacac aaggattaaa caaattagga ttaattacca ctgaagaatt gaatttgatt
     2761 catcaaggat tagaacaaat cagacaagaa tggcatgata ataaattcat tattaaggct
     2821 ggtgatgagg atattcatac tgctaatgaa cgtcgattag gagaaatcat tgggaaaaac
     2881 atttctggta aagttcacac tggtagatca agaaatgatc aagttgctac cgatatgaga
     2941 attttcgttc gtgaatcatt acttaattta agtaaaattt tacatcaatt catcacggca
     3001 attcttgaac gagcacataa agaaatcgat gtattaatgc ctggttatac tcatttacaa
     3061 aaagctcaac ctattagatg ggcccattgg ttaagttcat atgcaactta tttcaccgaa
     3121 gattataaac gtttacaaga aattataact cgtgttaacc aatctccatt aggatctggt
     3181 gctttagcag gtcatcctta tggtattgat cgtgaatttt tagctaaagg attaggattt
     3241 gatggtgtca ttgggaattc tttaactgca gtttcagatc gtgattttgt tgttgaaagt
     3301 ttattttgga gtacattatt tatgaatcat atttctcgat tctcagaaga tctaattatt
     3361 tattctagtg gtgaatttgg gttcataaaa ttagctgatg cttattctac tggttcttca
     3421 ttgatgccac aaaagaaaaa ccctgactca ttagaattat tacgtgggaa aagtggtaga
     3481 gtatttggtc agttatctgg atttttaatg tcgatcaaat ctattccatc tacttataat
     3541 aaagatatgc aagaggataa agaaccatta tttgatgcat tgactacagt agaacattca
     3601 attttaattg ctactggtgt gatttctact ttactgattg ataaacaaaa tatggaaaaa
     3661 gctttaacta tggatatgtt ggctactgat ttagctgatt atttagttag aaaaggagtt
     3721 ccatttagag aaactcatca tatatctggt gaatgtgtta gaaaagctga agaagaaaaa
     3781 ttatctggaa ttgatcaatt atcttttgaa caatttcaac aaattgattc tcgttttgaa
     3841 aaagacgtta tggaaacttt tgattttgaa gctagtgtgg aaagaagaga tgctcttggt
     3901 ggtactgcta aaagtgccgt tttaaaacaa ttagaaaatt taaaatcaat cttaagttaa
     3961 tttataaata gtcatataat aatcacagta ttgtttaact tacataaaat ataatcaatt
     4021 aacacagaga taccttgtac tacaatacta gcattccaac cagcatttct atacctgcta
     4081 gtgattggtc tggtgctagc aatatttatg agaaactatc acttctttta aactctacaa
     4141 tttgatatct cgagtaccaa tatatcggtt gcaccagctt tcttcaattc gtccattaca
     4201 ttaccgattt cctttctatt aaccatggat gagatggcaa cccagtcctc ttcgtcgtcg
     4261 ctgtgtttgt ccaaagtaga gacagtagcg gcccttctgc ctggagtaat ggttaaacat
     4321 tttgcttgga tggattttgg ggcattgtag ttacacaaga catattcctg ggcagctaaa
     4381 acaccttgaa gtctttggac gattatattg accatttctg ggaatttact tttctttgac
     4441 gaaatcaaat gagccgaagt ctccaatatg gtttctatgg cctttaaccc agctgctttc
     4501 atagtttcac cactttcaac caaatcgaca atagcatcag caacacccaa ggcacaagaa
     4561 gcctcaacgg aaccaccgac atatctgata ttagtaggtt tgtctgacaa ttgtttgaaa
     4621 tagtcggtac tcaattttgt aaatgaagac acaattttct ttccaacaag ctgttctggc
     4681 ttttcgtact cgccatctgc tggaacttgg atctgcaatt tgcatgaacc aaatttcaaa
     4741 tccaacaagt cctcgatgtt gtcgaattgt tcagcttctt tgatttggtc taacccagtt
     4801 atacccaagt cacaattacc ttctccaacg aaaactggga tatcagctgc aggcaagaag
     4861 attaatgcaa ttggcaagtt tgtagaaagt gctatatcta atctattaga tcttctaaac
     4921 tgtatatcgg caccactcaa taagttacag catttttcgt ataatctgcc ctttttagga
     4981 acagcaaata acaaacggtc tggtaaatga ttgactaaat ccattatcgg tagttggtgg
     5041 ttaagtaaaa gaagaagttg tgtgggaaaa atcttcacca aaaaaaaata aaaaatttca
     5101 atggttgcgt aataatcaaa atgagtcaat tctcacaacc gctcgcgaca cgtttcaacg
     5161 aaatggcctc ccctaccaca gaaaattaag tacacatgtt gtgatagatt ttacatttat
     5221 aaaaaactat taaataaata aattgtcttg ctgtcctatt gctttttgac tataccttcg
     5281 ctgtcttctt cctctgatgt cactgtatcc tcttctgtcc ccaaattgtt actactaact
     5341 tctgtctcct catcctccag gtaccggatc ccccagatca ttatccttaa acagattcta
     5401 aaacaacatc tttgaaacca gaagaaactt tcttaccttt ttcttcggtt gaaccttcac
     5461 cgtgcaaagc caataatgaa ccccagtcga attttggttg tttcaacaat ttgacttttc
     5521 tgatgtggac attttgtaat gggaaaatgg tttgggggga tttttcaatt tcacggccaa
     5581 tgacttctgg aatcaatttg gaagttaatt gagctaaagt cagttggaaa cttctctttg
     5641 cataatttca atcatcttct ttctgacttc tctcaattta gaggattgag cgtaagtagt
     5701 ttccttgatt tggtttggtt gtcttttggt gaaagcaatg gcaaaaactc tcaaaacgta
     5761 atcgtcggaa gttttaacag tgacattagc ttcaactaat gattgccatt ttctgaccaa
     5821 tgatcttaat ttgtcagaag tgaaatccaa accatgaggc ctccatgagg cctccatgag
     5881 gcctccatga ggcctccatg gaggcctcat ggaagttggt caacaagttt ttaccttgga
     5941 cttcatcaac tctcaatttg atttttctgt aagagtggtc ttcggaacct tgtaagtcgg
     6001 ccaaacaaac ttcgaaaact ctacctttca agccatcagc ggcattcttt aaaccggtag
     6061 atctgttgat caaagtttta ccaacatttc tgttttcaaa agtggttgga gctttgatgt
     6121 caaaccaatc ttttctggtg aatgggtcaa cgaccttctt ttttaatcct ttctttcctt
     6181 tggacaatct cttgttttta ccgacagcca tgttgtactt gagttggatc tacgcgtgaa
     6241 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccagcttttg
     6301 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     6361 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6421 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6481 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6541 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7801 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8101 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8461 ggttccgcgc acatttcccc gaaaagtgcc ac
//
