Basic Vector Information
- Vector Name:
- CIp20
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6481 bp
- Type:
- Candida integrating vector
- Replication origin:
- ori
- Source/Author:
- Dennison PM, Ramsdale M, Manson CL, Brown AJ.
- Promoter:
- T3
CIp20 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CIp20 vector Sequence
LOCUS 40924_475 6481 bp DNA circular SYN 17-DEC-2018 DEFINITION Candida integrating vector CIp20, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6481) AUTHORS Dennison PM, Ramsdale M, Manson CL, Brown AJ. TITLE Gene disruption in Candida albicans using a synthetic, codon-optimised Cre-loxP system JOURNAL Fungal Genet. Biol. 42 (9), 737-748 (2005) PUBMED 16043373 REFERENCE 2 (bases 1 to 6481) AUTHORS Dennison PMJ. TITLE Direct Submission JOURNAL Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS, Foresterhill, Aberdeen AB25 2ZD, UK REFERENCE 3 (bases 1 to 6481) TITLE Direct Submission REFERENCE 4 (bases 1 to 6481) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Genet. Biol."; date: "2005"; volume: "42"; issue: "9"; pages: "737-748" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS, Foresterhill, Aberdeen AB25 2ZD, UK" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6481 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1124..1933 /gene="URA3" /label=URA3 /note="Orotidine 5'-phosphate decarboxylase from Candida albicans (strain SC5314 / ATCC MYA-2876). Accession#: P13649" CDS complement(2120..3013) /gene="HIS1" /label=HIS1 /note="ATP phosphoribosyltransferase from Candida albicans (strain SC5314 / ATCC MYA-2876). Accession#: P46586" misc_feature 3361..4221 /label=RPS1 /note="RPS1" primer_bind complement(4247..4263) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(4293..4311) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4332..4348) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4356..4372) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4380..4410) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4425..4446) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4734..5322) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5496..6353) /label=AmpR /note="beta-lactamase" promoter complement(6354..6458) /label=AmpR promoter
This page is informational only.