CIp20 vector (V010038)

LOCUS       V010038                 6481 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V010038
VERSION     V010038
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Dennison PM, Ramsdale M, Manson CL, Brown AJ.
  TITLE     Gene disruption in Candida albicans using a synthetic,
            codon-optimised Cre-loxP system
  JOURNAL   Fungal Genet. Biol. 42 (9), 737-748 (2005)
   PUBMED   16043373
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Dennison PMJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS,
            Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE   3  (bases 1 to 6481)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal
            Genet. Biol."; date: "2005"; volume: "42"; issue: "9"; pages:
            "737-748"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2005) Medical Sciences, Aberdeen University, IMS,
            Foresterhill, Aberdeen AB25 2ZD, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     CDS             complement(2120..3013)
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     misc_feature    3361..4221
                     /label="RPS1"
                     /note="RPS1"
     primer_bind     complement(4247..4263)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4293..4311)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4332..4348)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4356..4372)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4380..4410)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4425..4446)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4734..5322)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5496..6353)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6354..6458)
                     /label="AmpR promoter"