LOCUS       V010038                 6481 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V010038
VERSION     V010038
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Dennison PM, Ramsdale M, Manson CL, Brown AJ.
  TITLE     Gene disruption in Candida albicans using a synthetic,
            codon-optimised Cre-loxP system
  JOURNAL   Fungal Genet. Biol. 42 (9), 737-748 (2005)
   PUBMED   16043373
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Dennison PMJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS,
            Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE   3  (bases 1 to 6481)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal
            Genet. Biol."; date: "2005"; volume: "42"; issue: "9"; pages:
            "737-748"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2005) Medical Sciences, Aberdeen University, IMS,
            Foresterhill, Aberdeen AB25 2ZD, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     CDS             complement(2120..3013)
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     misc_feature    3361..4221
                     /label="RPS1"
                     /note="RPS1"
     primer_bind     complement(4247..4263)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4293..4311)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4332..4348)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4356..4372)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4380..4410)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4425..4446)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4734..5322)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5496..6353)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6354..6458)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcggcc gacttggcca agcctagatc ccgactaata ggaattgatt
      721 tggatggtat aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta
      781 ttatttgatt ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag
      841 ttgctgtagt gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat
      901 agtatccaaa aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac
      961 ataatgaaag aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa
     1021 acattgatca attcctgagc aacaacccca tacacactgg tttatatacc gcccctttta
     1081 cagttgaaga aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa
     1141 gacctatagt gagagagcag aaactcatgc ctcaccagta gcacagcgat tatttcgatt
     1201 aatggaactg aagaaaacca atttatgtgc atcaattgac gttgatacca ctaaggaatt
     1261 ccttgaatta attgataaat taggtcctta tgtatgctta atcaagactc atattgatat
     1321 aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca
     1381 tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg taaagaaaca
     1441 atatattggt ggagtttata aaattagtag ttgggcagat attaccaatg ctcatggtgt
     1501 cactgggaat ggagtggttg aaggattaaa acagggagct aaagaaacca ccaccaacca
     1561 agagccaaga gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg
     1621 agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt
     1681 tattgcccaa cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc
     1741 tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga
     1801 agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag
     1861 agatccagat attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa
     1921 gactggccaa ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt
     1981 agaataaata aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt
     2041 tacaatcaaa ggtggtcctt ctagaactag tgattggtct ggtgctagca atatttatga
     2101 gaaactatca cttcttttaa actctacaat ttgatatctc gagtaccaat atatcggttg
     2161 caccagcttt cttcaattcg tccattacat taccgatttc ctttctatta accatggatg
     2221 agatggcaac ccagtcctct tcgtcgtcgc tgtgtttgtc caaagtagag acagtagcgg
     2281 cccttctgcc tggagtaatg gttaaacatt ttgcttggat ggattttggg gcattgtagt
     2341 tacacaagac atattcctgg gcagctaaaa caccttgaag tctttggacg attatattga
     2401 ccatttctgg gaatttactt ttctttgacg aaatcaaatg agccgaagtc tccaatatgg
     2461 tttctatggc ctttaaccca gctgctttca tagtttcacc actttcaacc aaatcgacaa
     2521 tagcatcagc aacacccaag gcacaagaag cctcaacgga accaccgaca tatctgatat
     2581 tagtaggttt gtctgacaat tgtttgaaat agtcggtact caattttgta aatgaagaca
     2641 caattttctt tccaacaagc tgttctggct tttcgtactc gccatctgct ggaacttgga
     2701 tctgcaattt gcatgaacca aatttcaaat ccaacaagtc ctcgatgttg tcgaattgtt
     2761 cagcttcttt gatttggtct aacccagtta tacccaagtc acaattacct tctccaacga
     2821 aaactgggat atcagctgca ggcaagaaga ttaatgcaat tggcaagttt gtagaaagtg
     2881 ctatatctaa tctattagat cttctaaact gtatatcggc accactcaat aagttacagc
     2941 atttttcgta taatctgccc tttttaggaa cagcaaataa caaacggtct ggtaaatgat
     3001 tgactaaatc cattatcggt agttggtggt taagtaaaag aagaagttgt gtgggaaaaa
     3061 tcttcaccaa aaaaaaataa aaaatttcaa tggttgcgta ataatcaaaa tgagtcaatt
     3121 ctcacaaccg ctcgcgacac gtttcaacga aatggcctcc cctaccacag aaaattaagt
     3181 acacatgttg tgatagattt tacatttata aaaaactatt aaataaataa attgtcttgc
     3241 tgtcctattg ctttttgact ataccttcgc tgtcttcttc ctctgatgtc actgtatcct
     3301 cttctgtccc caaattgtta ctactaactt ctgtctcctc atcctccagg taccggatcc
     3361 cccagatcat tatccttaaa cagattctaa aacaacatct ttgaaaccag aagaaacttt
     3421 cttacctttt tcttcggttg aaccttcacc gtgcaaagcc aataatgaac cccagtcgaa
     3481 ttttggttgt ttcaacaatt tgacttttct gatgtggaca ttttgtaatg ggaaaatggt
     3541 ttggggggat ttttcaattt cacggccaat gacttctgga atcaatttgg aagttaattg
     3601 agctaaagtc agttggaaac ttctctttgc ataatttcaa tcatcttctt tctgacttct
     3661 ctcaatttag aggattgagc gtaagtagtt tccttgattt ggtttggttg tcttttggtg
     3721 aaagcaatgg caaaaactct caaaacgtaa tcgtcggaag ttttaacagt gacattagct
     3781 tcaactaatg attgccattt tctgaccaat gatcttaatt tgtcagaagt gaaatccaaa
     3841 ccatgaggcc tccatgaggc ctccatgagg cctccatgag gcctccatgg aggcctcatg
     3901 gaagttggtc aacaagtttt taccttggac ttcatcaact ctcaatttga tttttctgta
     3961 agagtggtct tcggaacctt gtaagtcggc caaacaaact tcgaaaactc tacctttcaa
     4021 gccatcagcg gcattcttta aaccggtaga tctgttgatc aaagttttac caacatttct
     4081 gttttcaaaa gtggttggag ctttgatgtc aaaccaatct tttctggtga atgggtcaac
     4141 gaccttcttt tttaatcctt tctttccttt ggacaatctc ttgtttttac cgacagccat
     4201 gttgtacttg agttggatct acgcgtgaat tcgatatcaa gcttatcgat accgtcgacc
     4261 tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     4321 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4381 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4501 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4561 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4621 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4681 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4741 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5161 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5221 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5281 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5341 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5401 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5461 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5521 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5581 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5641 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5701 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5761 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5821 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5881 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5941 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6001 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6061 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6121 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6181 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6241 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6301 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6361 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6421 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6481 c
//
