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pMET B vector (V010057)

Basic Vector Information

      • Vector Name:
      • pMET B
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7779 bp
      • Type:
      • Yeast Expression Vectors
      • Replication origin:
      • ori
      • Selection Marker:
      • ADE2
      • Copy Number:
      • High copy number
      • Promoter:
      • ADE2
      • Cloning Method:
      • Enzyme digestion and ligation
      • 5' Primer:
      • AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´
      • 3' Primer:
      • AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
      • Fusion Tag:
      • C-V5, C-His

pMET B vector Vector Map

pMET B7779 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500V5 tag6xHisADE2 promoteroriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMET B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_30700        7779 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7779)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1269..1310
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1320..1337
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        2445..2820
                     /label=ADE2 promoter
     rep_origin      complement(5751..6339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6513..7370)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7371..7475)
                     /label=AmpR promoter

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