LOCUS       40924_30700        7779 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1269..1310
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1320..1337
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        2445..2820
                     /label=ADE2 promoter
     rep_origin      complement(5751..6339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6513..7370)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7371..7475)
                     /label=AmpR promoter
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcgagagga
     1201 attcacgtgg cccagccggc cgtggatcca cgcgtcgtcg acccgcggcg gccgccagct
     1261 tactagtagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
     1321 atcatcacca tcaccattga tctagtatac aattctaggg ctgcctgttt ggatattttt
     1381 ataatttttg agagtttgcc aactaatgtt tttctcttct atgatattta tcatgtagtt
     1441 ggatatcttt ttttctttga aaagttttta tttagttctt tctctctttt ttataatgtt
     1501 ttcagtttct ttcctttata ttgaaaatac gatgttcttt gtctttcttt tttaaatcct
     1561 caattataat gtgtaccgtt tgatcaactt gtaaaattgt ttgtagtcct gtaatcttaa
     1621 ttgacgattg gttgatagtt taccccagta tattgtgcgg ctgagatatt acccgattcg
     1681 gaagattgat tttttcaaca gacataaacc cataggagat ttagtacagc gacatatgct
     1741 aattgcctga taatgctcac taacattttc tccttttaat ctggtatatg catacaggta
     1801 tgttgatatg gccttgttga tcaaagctac gaaagtgtag actatttccc aaacagcccc
     1861 caaccaccac ctcacacgcg cacactgcga agtgaaagcg ctcccttatt ttcttctccg
     1921 ctggttcaaa acaaaaaaga atctaatctg tagacacacg ccccgtaaaa cccaatcttc
     1981 atcatcgtaa ttttcagtga cggacgatcg atcaaccata tagtttgtta gtgactgagc
     2041 ttcagtagtt gacctcttga agaaataacg tcttgaacaa catactttat acctaccaac
     2101 attcgccatc aacaacagtg tgacactgac ataactttac atccattcat cgacaaaaaa
     2161 atgtctctag ttcggattca tgcttatggg ttagctattt cgcccaatgt gtccatctga
     2221 cattactatt ttgcatttta atttaattag aacttgacta gcgcactacc agcatatcat
     2281 ctcatttccg taaataccaa atgtattata tattgaaagc ttttgaccag gttattataa
     2341 aagaaacttc atgctcgaaa aagatcattt cgaaaagttg cctagtttca tgaaatttta
     2401 aagcagttta tataaatttt accttttgat gcggaattga ctttttcttg aataatacat
     2461 aacttttctt aaaagaatca aagacagata aaatttaaga gatattaaat attagtgaga
     2521 agccgagaat tttgtaacac caacataaca ctgacatctt taacaacttt taattatgat
     2581 acatttctta cgtcatgatt gattattaca gctatgctga caaatgactc ttgttgcatg
     2641 gctacgaacc gggtaatact aagtgattga ctcttgctga ccttttatta agaactaaat
     2701 ggacaatatt atggagcatt tcatgtataa attggtgcgt aaaatcgttg gatctctctt
     2761 ctaagtacat cctactataa caatcaagaa aaacaagaaa atcggacaaa acaatcaagt
     2821 atggattcta gaacagttgg tatattagga gggggacaat tgggacgtat gattgttgag
     2881 gcagcaaaca ggctcaacat taagacggta atactagatg ctgaaaattc tcctgccaaa
     2941 caaataagca actccaatga ccacgttaat ggctcctttt ccaatcctct tgatatcgaa
     3001 aaactagctg aaaaatgtga tgtgctaacg attgagattg agcatgttga tgttcctaca
     3061 ctaaagaatc ttcaagtaaa acatcccaaa ttaaaaattt acccttctcc agaaacaatc
     3121 agattgatac aagacaaata tattcaaaaa gagcatttaa tcaaaaatgg tatagcagtt
     3181 acccaaagtg ttcctgtgga acaagccagt gagacgtccc tattgaatgt tggaagagat
     3241 ttgggttttc cattcgtctt gaagtcgagg actttggcat acgatggaag aggtaacttc
     3301 gttgtaaaga ataaggaaat gattccggaa gctttggaag tactgaagga tcgtcctttg
     3361 tcgccgaaaa atgggcacca tttactaaag aattagcagt catgattgtg agatctgtta
     3421 acggtttagt gttttcttac ccaattgtag agactatcca caaggacaat atttgtgact
     3481 tatgttatgc gcctgctaga gttccggact ccgttcaact taaggcgaag ttgttggcag
     3541 aaaatgcaat caaatctttt cccggttgtg gtatatttgg tgtggaaatg ttctatttag
     3601 aaacagggga attgcttatt aacgaaattg ccccaaggcc tcacaactct ggacattata
     3661 ccattgatgc ttgcgtcact tctcaatttg aagctcattt gagatcaata ttggatttgc
     3721 caatgccaaa gaatttcaca tctttctcca ccattacaac gaacgccatt atgctaaatg
     3781 ttcttggaga caaacataca aaagataaag agctagaaac ttgcgaaaga gcattggcga
     3841 ctccaggttc ctcagtgtac ttatatggaa aagagtctag acctaacaga aaagtaggtc
     3901 acataaatat tattgcctcc agtatggcgg aatgtgaaca aaggctgaac tacattacag
     3961 gtagaactga tattccaatc aaaatctctg tcgctcaaaa gttggacttg gaagcaatgg
     4021 tcaaaccatt ggttggaatc atcatgggat cagactctga cttgccggta atgtctgccg
     4081 catgtgcggt tttaaaagat tttggcgttc catttgaagt gacaatagtc tctgctcata
     4141 gaactccaca taggatgtca gcatatgcta tttccgcaag caagcgtgga attaaaacaa
     4201 ttatcgctgg agctggtggg gctgctcact tgccaggtat ggtggctgca atgacaccac
     4261 ttcctgtcat cggtgtgccc gtaaaaggtt cttgtctaga tggagtagat tctttacatt
     4321 caattgtgca aatgcctaga ggtgttccag tagctaccgt cgctattaat aatagtacga
     4381 acgctgcgct gttggctgtc agactgcttg gcgcttatga ttcaagttat acaacgaaaa
     4441 tggaacagtt tttattaaag caagaagaag aagttcttgt caaagcacaa aagttagaaa
     4501 ctgtcggtta cgaagcttat ctagaaaaca agtaatatat aagtttattg atatacttgt
     4561 acagcaaata attataaaat gatataccta ttttttaggc tttgttatga ttacatcaaa
     4621 tgtggacttc atacatagaa atcaacgctt acaggtgtcc ttttttaaga atttcataca
     4681 taagatctac tagaatcaat gttaaacctg ttatcattag aacagccatc tccagaaggt
     4741 tctatccaag tgaaaacttt ggtcttcaag ccaaagactg ctaaaacaca aaccccagtc
     4801 ccaattattg ttgttgctga aacttctact tctactccgg gtggtgttat agccaaagct
     4861 tcgggcacta aagagccaag attagcaaac gatgaattga ccaaccaatt ctttggttct
     4921 cctgctaaag agttttctat tgccagctta tcaaacgaac acaaagacgg taaagtcaag
     4981 atcattattg atgctgggat caaagatgat gactcgacta attacgttat ttccaacact
     5041 gttgctaagt tagttttaac cggtaagcaa ttattaggtt tcttatccaa ctacgaacca
     5101 accatcgttg attttactgc tgaagctgca accgctgccc caactccagt tgttaagaaa
     5161 gaacctgctg catctggtaa gcttgatgat gccaaattaa ttggtgttac tgctgacaag
     5221 gaaaaagatt tctccaactg gtactcccaa attctaatga agggtgaaat gttagactac
     5281 tacgatgtct ctggttgtta cattatgaga ccagcttctt acacaatttg ggaaaagatt
     5341 caatcttggt tcgattcgaa gattaaagaa gatggtgtta ctaatgctta tttcccaatg
     5401 tttgtctcat ccaaggtttt agaaaaggaa aaggaccaca tcgatggttt cgctcctgaa
     5461 gttgcttggg ttaccagagc tggtaaatct gaattagaag aaccaattgc tattagacca
     5521 acttctgaaa ctgttatggg taccctgcag gggccggcct taattaaggc gcgcccttcc
     5581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5821 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5881 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5941 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6001 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6061 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6361 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7501 gtatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     7561 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7621 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     7681 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     7741 agggcgaaaa accgtctatc aagggcgatg gcccactac
//
