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B vector

Basic Vector Information

Basic Vector Information
Vector Name B Antibiotic Resistance Ampicillin
Length 5830 bp Type YTT vector
Source Wilson CJ, Guglielmo C, Moua ND, Tudor M, Grosveld G, Young RA, Murray PJ.

B vector Vector Map

B vector Sequence

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LOCUS       Exported                5830 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  YTT vector B, complete sequence.
ACCESSION   AY028413
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Wilson CJ, Guglielmo C, Moua ND, Tudor M, Grosveld G, Young RA, 
            Murray PJ.
  TITLE     YAC Targeting Technology (YTT): An approach for the deletion of 
            genes in the C57BL/6 mouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Wilson CJ, Murray PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Infectious Diseases, St. Jude Children's 
            Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
REFERENCE   3  (bases 1 to 5830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="YTT vector B"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:157153"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    424..885
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(901..1695)
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /protein_id="AAK29771.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(901..1695)
                     /gene="kanR"
                     /label=kanR
     regulatory      complement(1706..1786)
                     /regulatory_class="promoter"
                     /note="EM7"
     promoter        complement(1727..1774)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     regulatory      complement(1786..2310)
                     /regulatory_class="promoter"
                     /note="pgk"
     promoter        complement(1787..2283)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      2311..2707
                     /regulatory_class="promoter"
                     /note="TEF"
     promoter        2364..2707
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2708..3406
                     /codon_start=1
                     /gene="his3"
                     /product="His3"
                     /label=his3
                     /note="derived from Saccharomyces kluyveri"
                     /protein_id="AAK29770.1"
                     /translation="MTEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     gene            2708..3406
                     /gene="his3"
                     /label=his3
     regulatory      3408..3571
                     /regulatory_class="terminator"
                     /note="TEF"
     primer_bind     complement(3609..3625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3633..3649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3657..3687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3702..3723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4011..4599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4770..5630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5631..5735)
                     /gene="bla"
                     /label=AmpR promoter

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