Basic Vector Information
pMXs-Sox2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMXs-Sox2 vector Sequence
LOCUS 40924_32913 5766 bp DNA circular SYN 13-MAY-2021 DEFINITION Retroviral expression of mouse Sox2. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5766) AUTHORS Takahashi K, Yamanaka S TITLE Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. JOURNAL Cell. 2006 Aug 25. 126(4):663-76. PUBMED 16904174 REFERENCE 2 (bases 1 to 5766) TITLE Direct Submission REFERENCE 3 (bases 1 to 5766) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 Aug 25. 126(4):663-76." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5766 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(677..1534) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1535..1639) /label=AmpR promoter misc_feature 2325..2682 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2741..3157 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" misc_feature 3167..3541 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" protein_bind 3592..3616 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" CDS 3616..4572 /codon_start=1 /label=mSox2 /note="Mus musculus transcription factor SOX-2 gene. Belongs to the SRY-related HMG-box (SOX) family of transcription factors, which is involved in the regulation of embryonic development and in the determination of cell fate" /translation="LYNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKR PMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHM KEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYA HMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGS PTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLP GAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM" protein_bind complement(4577..4601) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" LTR 4796..5387 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" rep_origin complement(join(5688..5766,1..510)) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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