LOCUS       40924_32913        5766 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Retroviral expression of mouse Sox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 5766)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(677..1534)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1535..1639)
                     /label=AmpR promoter
     misc_feature    2325..2682
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             2741..3157
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    3167..3541
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     protein_bind    3592..3616
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3616..4572
                     /codon_start=1
                     /label=mSox2
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="LYNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKR
                     PMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHM
                     KEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYA
                     HMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGS
                     PTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLP
                     GAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM"
     protein_bind    complement(4577..4601)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             4796..5387
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(join(5688..5766,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
      661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc
     1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
     2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
     2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
     2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
     2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata
     2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
     2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
     2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt
     3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
     3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
     3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
     3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
     3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
     3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact
     3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaaat cacaagtttg
     3601 tacaaaaaag caggcttgta taacatgatg gagacggagc tgaagccgcc gggcccgcag
     3661 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac
     3721 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg
     3781 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag
     3841 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag
     3901 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg
     3961 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc
     4021 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg
     4081 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg
     4141 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag
     4201 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg
     4261 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc
     4321 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc
     4381 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg
     4441 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac
     4501 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc
     4561 ctgtcgcaca tgtgaaaccc agctttcttg tacaaagtgg tgatattcca gctgagcgcc
     4621 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     4681 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaactcgag cggccgccag
     4741 cacagtggtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     4801 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4861 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     4921 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4981 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5041 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     5101 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     5161 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     5221 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     5281 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     5341 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     5401 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     5461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5581 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5761 ggacta
//
