AtGH3-LUC-NOS-At2g23710 vector (V010108)

Basic Vector Information

      • Vector Name:
      • AtGH3-LUC-NOS-At2g23710
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8247 bp
      • Type:
      • Control vector
      • Source/Author:
      • Kovtun Y, Chiu WL, Tena G, Sheen J.

AtGH3-LUC-NOS-At2g23710 vector Vector Map

AtGH3-LUC-NOS-At2g237108247 bp400800120016002000240028003200360040004400480052005600600064006800720076008000cloning vectorAtGH3 enhancer/promoterLUCNOS terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AtGH3-LUC-NOS-At2g23710 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8247 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector AtGH3-LUC-NOS-At2g23710, complete sequence.
ACCESSION   EF090410
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Hwang I, Sheen J.
  TITLE     Two-component circuitry in Arabidopsis cytokinin signal transduction
  JOURNAL   Nature 413 (6854), 383-389 (2001)
  PUBMED    11574878
REFERENCE   3  (bases 1 to 8247)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 8247)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Control vector AtGH3-LUC-NOS-At2g23710"
                     /mol_type="other DNA"
                     /label=derived from Arabidopsis thaliana
                     /note="derived from Arabidopsis thaliana"
                     /db_xref="taxon:412980"
     misc_feature    1..2666
                     /label=cloning vector
                     /note="cloning vector"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1176..2036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2037..2141)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..6183
                     /regulatory_class="enhancer"
                     /note="AtGH3 enhancer/promoter"
     CDS             6184..7836
                     /codon_start=1
                     /product="luciferase"
                     /label=luciferase
                     /note="LUC"
                     /protein_id="ABL09839.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     terminator      7991..8242
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      7994..8242
                     /regulatory_class="terminator"
                     /note="NOS terminator"

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