LOCUS       40924_310        8247 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector AtGH3-LUC-NOS-At2g23710, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Hwang I, Sheen J.
  TITLE     Two-component circuitry in Arabidopsis cytokinin signal transduction
  JOURNAL   Nature 413 (6854), 383-389 (2001)
  PUBMED    11574878
REFERENCE   3  (bases 1 to 8247)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 8247)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8247)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: 
            "2940-2945"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nature"; 
            date: "2001"; volume: "413"; issue: "6854"; pages: "383-389"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..6183
                     /label=AtGH3 enhancer/promoter
                     /note="AtGH3 enhancer/promoter"
                     /regulatory_class="enhancer"
     CDS             6184..7833
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7991..8242
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatccttgt cttgcgcatg gagatatcaa cagtggtctt
     2701 aaagactatt attgacaaca agtcaacaaa cattaaacgt gcacctgtct actaactact
     2761 aataataata atgttaatga caaaaagata attacaacat ccaatcttcg aattatttag
     2821 aagaaacatc actttgaact tttgaagtat ataaagaaaa taatgctctt ttatctttct
     2881 ttaatttctt gatatttaaa tattataatc aaataacaat tgcggatgtt aattgtataa
     2941 tactctcctc caaactagca ggcacttagt caagatttct taatgttatt taatcgcatg
     3001 gttgagaaaa aataaataat aataataata ataatataca gctatagaat tattgtttat
     3061 ctagaaaaca atttttgggt taaacattcg gttaacttgg gtatgaaatg gtatatatat
     3121 tgttttagca ttttgaatca atataaagaa aacataattc tacgtatcca aattctctct
     3181 caaaatcttc gaccgaatta acgcaaacct tgaaccattc atcatttccc tatgaatgta
     3241 atcaaaacag caatggagtt aattttgtac tatttattaa aaagttatgg aacatacttg
     3301 aattgcttaa gattctaaag aaaatatctg tcggattata gtggccattc tttcttttct
     3361 tttatttttg caataattag tggccatgcc ttttccatgc ttgatattga aagttgattc
     3421 acaactcgta aaatatatct ctccgaacgc agcttcgtct ttgaattttg aaatagtttc
     3481 ttctaatcaa caaaacaaaa aaacttttat agataatttg gttttagttt tacataatac
     3541 catttaacat cgagtaatga gctcaattag acaaagataa tatactgtat tatttagacc
     3601 aagaaaatta gtaacgaatt tctaacctat tcaagtaaaa taacagacaa gtgggaggaa
     3661 ataactattt caattaacgt atgcaatact aaggaatagt ggtatttaat tattaaaaag
     3721 aagaagaaag aatagtgata tggacatact agaaaattgt tgaaatggtc caagtgttcc
     3781 cctaaatgtc taatcaaaga taaggcatga cccgtgcgac cagtcacttc ctcaatttgt
     3841 tttctttctc tcattaggtc atattcatat gcataccgtc tttttatcaa ttacatattc
     3901 aataattatt gttttaggtc gtatattctc tttttctatt agtattatgt ataccagtaa
     3961 tcttatttag tctttatttt aaggcttaca tgtcaactga tcgccatgac tgaatattat
     4021 cttcggatac ctccaagata tcaaataata taatatttga ataatattaa tatcataatt
     4081 ctgatttttt tgtatataaa gcaaaaggat acaaatgtat atgcttttca tattttcatc
     4141 tatgtgatgt atattattga tttttcttac caagatacca ccgtattaat ttttatatct
     4201 tattatttat tactggtatc gtaagaccaa tctctttaac acatcgccaa aaaaaatgta
     4261 taagagctac attatcagtt ggcttggatt ttcctttttt ctgccttcat cactttgcgc
     4321 gttaattcct tctataatcg tcctcaaata tttttaaatt gctatgtgct gaatatttta
     4381 aattttcata aactttaaag caacctaata attttctatt tctttcaata tatttattgt
     4441 ttttcctatt taccaattta aataaaatat aattttaata cataatatac attaaaaatt
     4501 gaacctcttg ctctttcaga agaaaaaaaa caaaaagcaa aaaccctaga attaaatagt
     4561 actgtcaagt tggagtgggt acaggcacga tcgagcaaaa cgaaatgtcc catgcatgcc
     4621 tttaattgct ttagagttag ttcttccaaa tcaagaatta gttttagtta tcaataaata
     4681 ggagtaccca ctagtagtcg taccacgacc tttcaattca taggacaaac gtcttacccc
     4741 ctgcattatt atgcgatata tatgtgaatc atacatcata catggtgcct aaagaaaatg
     4801 tgaatcatac atatgaatct taattattcc atacatttaa attatgaaat gcataaaaat
     4861 gttttcgaaa atgattttat aaacatatac gcagaatctc aaagttgcta attgtatact
     4921 attgctttgg tttggttgtt aggctaacgt cccatgatcc ttcggaaaga taaatatgca
     4981 tgacccggag tgatggagca tctaaaattt tatggaagct ttgctcttaa aaagattaag
     5041 ctgtaattgt ataagtatat atagttttaa ccaacttgtg gttatttttg tagtcgtcga
     5101 aatatttatt atgggattgg aagatcatgg tggattgata tgtgtgtctt ttctatatat
     5161 ttttaatatt taggtcccat taaatcagtt tgtgatttca gaatcctaac ataccctttt
     5221 gagattactt ttcctattta tctcgaacat aacctcattc taaaactaca acactctatt
     5281 gggacaattc tatttggatc aaatttgtgc tatagtcatg aatttattac tcaaaattaa
     5341 ctaaaaagaa atgaacgaca catattttaa atgttttcta ggtttaatat caacttcaga
     5401 tgtggaatat tccaaacatg ttcgagtttt tctattgcaa tttcaataat aattgatcat
     5461 tgtggacgtt ttaaatcact agaacatttt ggcctctcac atgtttcatg attagtcttt
     5521 attttatata cagaattccg gattatgaga gaaaaaaaca catactttta ttaataatta
     5581 caaatttaaa ttgaaaattt atctttatgt tattacatcc aatgaactaa tgtcaatgca
     5641 tggaaagaat tacttaggct ccgggaacaa caacatgatc ccttcacacg catactctaa
     5701 ttcaactaac caagtcttct taattctgat aagtcaaatt gaaaatgtca ttaccactga
     5761 ttaaaaagga atcaaaagaa aaaaagcttt tagtattgag tattgaccgt cgctatcggt
     5821 gacaggcaga gtcacaagcc aaataaaagg gaaccgcgtg gtacgtacgg gctgacgcct
     5881 agctgctacg gtcatgacaa taaattgccc aatcaaagta acatgccaac gtggcgcaga
     5941 catatcagtc ccacatgtct gcccaaaact agccaaagat tacgtgaccg cggtccctct
     6001 tgtcccctgt ctcggtctaa cgataacaaa ccgagcccac ttttatgtcg acgtggaatt
     6061 tggctgacgt tggttttctc cttcttgcca ctataaatac aaccccatac tcgtcgagtt
     6121 tcaatatctc ctcatcatca aacacaaagt ctaatattat cacttacaaa taccatttta
     6181 tccatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat
     6241 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     6301 attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg
     6361 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     6421 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     6481 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac
     6541 atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     6601 caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag
     6661 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     6721 gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct
     6781 ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc
     6841 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     6901 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     6961 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat
     7021 tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact
     7081 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt
     7141 tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga
     7201 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     7261 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     7321 aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc
     7381 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     7441 tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag
     7501 tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta
     7561 caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa
     7621 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     7681 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     7741 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     7801 ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac
     7861 gaaattctta gctattgtaa tattatatgc aaattgatga atggtaattt tgtaattgtg
     7921 ggtcactgta ctattttaac gaataataaa atcaggtata ggtaactaaa aaggaattcg
     7981 agctcgaatt gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     8041 cggtcttgcg atgattatca tataatttct gttgattacg ttaagcatgt aataattaac
     8101 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     8161 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     8221 gtgtcatcta tgttactaga tctctag
//
