Basic Vector Information
- Vector Name:
- ASM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4791 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard SG.
- Promoter:
- T3
ASM vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ASM vector Sequence
LOCUS 40924_300 4791 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector ASM, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4791)
AUTHORS Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard
SG.
TITLE Plasmid vectors for proteomic analyses in giardia: purification of
virulence factors and analysis of the proteasome
JOURNAL Eukaryotic Cell 11 (7), 864-873 (2012)
PUBMED 22611020
REFERENCE 2 (bases 1 to 4791)
AUTHORS Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard
SG.
TITLE Direct Submission
JOURNAL Submitted (09-MAR-2012) Department of Cell and Molecular Biology,
Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE 3 (bases 1 to 4791)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4791)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
Cell"; date: "2012"; volume: "11"; issue: "7"; pages: "864-873"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-MAR-2012) Department of Cell and Molecular Biology, Uppsala
University, BMC, Box 596, Uppsala 751 24, Sweden"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4791
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 24..128
/label=AmpR promoter
CDS 129..986
/label=AmpR
/note="beta-lactamase"
rep_origin 1160..1748
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2036..2057
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2072..2102
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2110..2126
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2134..2150
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2171..2189
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS complement(2334..2927)
/label=PuroR
/note="puromycin N-acetyltransferase"
5'UTR 2934..2981
/note="glutamate dehydrogenase 5' UTR; derived from Giardia
intestinalis"
5'UTR 2988..3187
/note="ornithine carbamoyltransferase 5' UTR; derived from
Giardia intestinalis"
CDS 3200..3313
/label=SBP
/note="streptavidin-binding peptide"
CDS 3320..3970
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
CDS 3977..4000
/label=HRV 3C site
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
CDS 4040..4057
/label=6xHis
/note="6xHis affinity tag"
3'UTR 4058..4133
/note="alpha-1 giardin 3' UTR; derived from Giardia
intestinalis"
promoter complement(4148..4166)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4176..4192)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4334..4789
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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