ASM vector (V010110)

LOCUS       40924_300        4791 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector ASM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard 
            SG.
  TITLE     Plasmid vectors for proteomic analyses in giardia: purification of 
            virulence factors and analysis of the proteasome
  JOURNAL   Eukaryotic Cell 11 (7), 864-873 (2012)
  PUBMED    22611020
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard 
            SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology, 
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2012"; volume: "11"; issue: "7"; pages: "864-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala 
            University, BMC, Box 596, Uppsala 751 24, Sweden"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2334..2927)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     5'UTR           2934..2981
                     /note="glutamate dehydrogenase 5' UTR; derived from Giardia
                     intestinalis"
     5'UTR           2988..3187
                     /note="ornithine carbamoyltransferase 5' UTR; derived from 
                     Giardia intestinalis"
     CDS             3200..3313
                     /label=SBP
                     /note="streptavidin-binding peptide"
     CDS             3320..3970
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             3977..4000
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             4040..4057
                     /label=6xHis
                     /note="6xHis affinity tag"
     3'UTR           4058..4133
                     /note="alpha-1 giardin 3' UTR; derived from Giardia 
                     intestinalis"
     promoter        complement(4148..4166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4176..4192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4334..4789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"