LOCUS       40924_300        4791 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector ASM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard 
            SG.
  TITLE     Plasmid vectors for proteomic analyses in giardia: purification of 
            virulence factors and analysis of the proteasome
  JOURNAL   Eukaryotic Cell 11 (7), 864-873 (2012)
  PUBMED    22611020
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Jerlstrom-Hultqvist J, Stadelmann B, Birkestedt S, Hellman U, Svard 
            SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology, 
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2012"; volume: "11"; issue: "7"; pages: "864-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala 
            University, BMC, Box 596, Uppsala 751 24, Sweden"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2334..2927)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     5'UTR           2934..2981
                     /note="glutamate dehydrogenase 5' UTR; derived from Giardia
                     intestinalis"
     5'UTR           2988..3187
                     /note="ornithine carbamoyltransferase 5' UTR; derived from 
                     Giardia intestinalis"
     CDS             3200..3313
                     /label=SBP
                     /note="streptavidin-binding peptide"
     CDS             3320..3970
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             3977..4000
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             4040..4057
                     /label=6xHis
                     /note="6xHis affinity tag"
     3'UTR           4058..4133
                     /note="alpha-1 giardin 3' UTR; derived from Giardia 
                     intestinalis"
     promoter        complement(4148..4166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4176..4192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4334..4789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccagc tgatcggcgc
     2221 cgggatgggg gtcctggggc agatagccgt tggaacggac agcgacttcc gcatcgtcaa
     2281 gcacgcccgt cgaacccgga aaaggtaaat attcacttca gccctcactc gagggcaccg
     2341 ggcttgcggg tcatgcacca ggtcgcgcgg tccttcgggc actcgacgtc ggcggtgacg
     2401 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     2461 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     2521 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     2581 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     2641 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     2701 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     2761 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg
     2821 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg
     2881 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtgcc catggatttt
     2941 aaaatctggg gcgcctgtaa ttaaaggcgt tatttgtggt caagcttgtc agcttcattt
     3001 ttattatcga ataacatcat tggagcgcag ggaatgctga cagggaaaga gtgctcatgt
     3061 gaatgaaccg actagagaag gaggggcaga cattaaagga gtattgacgt ttaagtgtcc
     3121 aattaattat ggataattca tttgtatacg ccaactaaac gctctacagt agaggctgaa
     3181 aattaaaatg ccggatatca tggacgagaa gaccaccggc tggcgcggcg gccacgtcgt
     3241 cgagggcctc gccggcgagc ttgagcagct cagggccagg cttgagcacc acccgcaggg
     3301 ccagagggag ccgatcgatt cccctatact aggttattgg aaaattaagg gccttgtgca
     3361 acccactcga cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg
     3421 cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct
     3481 tccttattat attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat
     3541 agctgacaag cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct
     3601 tgaaggagcg gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt
     3661 tgaaactctc aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga
     3721 tcgtttatgt cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt
     3781 gtatgacgct cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa
     3841 attagtttgt tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc
     3901 cagcaagtat atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca
     3961 tcctccaaaa tcggatctgg aagttctgtt ccaggggccc ctgggatcct gcagtctaga
     4021 cgcgtcgaat tcgcggccgc accaccacca ccaccactag gggtttagtg aacgtcttta
     4081 gctttgtctc tataactcta cggttaatca cggcgtctcc ccagaaatgc ggggagctcc
     4141 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4201 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4261 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4321 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4381 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4681 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4741 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta g
//
