Alpha-3000-gli2act vector (V010122)

Basic Vector Information

      • Vector Name:
      • Alpha-3000-gli2act
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 10802 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.

Alpha-3000-gli2act vector Vector Map

Alpha-3000-gli2act10802 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500Gli2actT7 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Alpha-3000-gli2act vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               10802 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Alpha-3000-gli2act, complete sequence.
ACCESSION   MF435040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Gli2 is required for the induction and migration of Xenopus laevis 
            neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San 
            Miguel de Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Cloning vector Alpha-3000-gli2act"
                     /mol_type="other DNA"
                     /db_xref="taxon:2052061"
     CDS             6..4067
                     /codon_start=1
                     /gene="Gli2 activator"
                     /product="Gli2act"
                     /label=Gli2 activator
                     /note="activator form of Gli2 protein, without the 
                     N-terminal repressor region"
                     /protein_id="ATQ62667.1"
                     /translation="MGGGALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGS
                     YGHLSAGAISPAFSFPIHKPCSLSAALSQQRSLSSSFGHTPLLHPSPTFASRQQGALTS
                     ANPAPPSNNSSAPDSVLNKVSSESAVSSTVNQVIHKRSKVKTEEEADSVRFPQPPDHLT
                     DLKEDLDKDECKQEPEHIYETNCHWDGCSKEFDTQDQLVHHINNDHIHGEKKEFVCRWQ
                     DCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCFKAYSRLENLKTHLRSHTGEKPYV
                     CDHEGCNKAFSNASDRAKHQNRTHSNEKPYICKVPGCTKRYTDPSSLRKHVKTVHGPEA
                     HVTKKHRNDIIQKPSLPKENGDNEASAKLSGREHSDSVSRDQEHCLQTRTIKTEDNMMH
                     QSSPGGQSSCSSEPSPYGNTNNIDSGVDVSLAWQGSLGDLFGLEETSPVVDSTVSSWQR
                     SGRPATPETQRIHSAETGTAEEREIKDNERFLLIYEPNATCQNTRLPTISANGFDVIGV
                     PSSVLINPRAIELSMNDVTMMNQLNERRDSTSSTLSSAYTSRRSSGISPYFSSRRSSET
                     SQFGGRLNNSSSADSYDPISTDASRRSSEASQHSGLPNLLNLTPAQHYRLKAKYAAATG
                     GPPPTPLPNMDRIGLRNKLSLMDGADFPLPPFRQLPVPRRCSDGGGNAGLTPMYPHEIP
                     GNNSRRASDPVRRTAGIDDKPLPRFSRFHSMNSMNTLHPPSLSERRNGGLQHYTCSDGG
                     LHRHVYSPRPPSISENVAMEAISCDADVPGGDDDLMLPDDVVQYIRSQNREAPEQNLQT
                     EYSSPARNLQSNTKSFHNNTPEQPRAPGAYLSRNFPALAECLGQTANMQDNNMPVQWNE
                     VSSGTVDVSDLPKQQFAAGNLAVVQQKQNFAQYQSFNQAPMQRAHNIMGQGQESVQRNI
                     SVNGQRFNYLQQRQQQMSQCQIVSSDFIPQQRYSQSQSMLSSRAMQEGQSQISPSCNNM
                     VERPGVHTHAAPSNTLNHQRLAVHGAPTQGFANNFSVNQDGLHPPNAYTVQPQKNGLEP
                     QQNTLGMSGQAFNHGMIQPRPPAAPHPPNRPRNIHPVHHPPYMRSPHPVSELSPGQQTA
                     EATPKRTSENTDPTPKDNNLLYYSGQIHMYEPNGNFGSGIDCTVRQLPTMPSPGANQVT
                     STVDSQGLEHPPVIDFDAMMDDGDHSSLMSGTLSPGLLQSFSQTSSRLTTPRNSLTLPS
                     IPAGINNMAIGDMSSMLTTLAEESKFLNLNRFKAMEQKLISEEDLNEMEQKLISEEDLN
                     EMEQKLISEEDLNEMEQKLISEEDLNEMEQKLISEEDLNEMESLGDLTMEQKLISEEDL
                     NSRPLEPLEL"
     gene            6..4067
                     /gene="Gli2 activator"
                     /label=Gli2 activator
     sig_peptide     3783..4037
                     /gene="Gli2 activator"
     CDS             3786..3815
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3825..3854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3864..3893
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3903..3932
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3942..3971
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4005..4034
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4062..4079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4084..4279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SV40 polyA"
     gene            4084..4279
                     /gene="SV40 polyA"
                     /label=SV40 polyA
     polyA_signal    4084..4218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4317..4335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4765..4781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4789..4805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4813..4843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4858..4879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5167..5755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5926..6786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6787..6891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7365..7381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7703..10787
                     /regulatory_class="promoter"
                     /gene="Xlp-Sla-3000"
     gene            7703..10787
                     /gene="Xlp-Sla-3000"
                     /label=Xlp-Sla-3000
     regulatory      10610..10615
                     /regulatory_class="TATA_box"
                     /gene="Xlp-Sla-3000"

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