LOCUS       40924_230       10802 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Alpha-3000-gli2act, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Gli2 is required for the induction and migration of Xenopus laevis 
            neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San 
            Miguel de Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 10802)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2017) Developmental Biology, Instituto Superior de 
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San 
            Miguel de Tucuman, Tucuman T4000ILI, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..4067
                     /codon_start=1
                     /gene="Gli2 activator"
                     /product="Gli2act"
                     /label=Gli2 activator
                     /note="activator form of Gli2 protein, without the
                     N-terminal repressor region"
                     /protein_id="ATQ62667.1"
                     /translation="MGGGALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASGS
                     YGHLSAGAISPAFSFPIHKPCSLSAALSQQRSLSSSFGHTPLLHPSPTFASRQQGALTS
                     ANPAPPSNNSSAPDSVLNKVSSESAVSSTVNQVIHKRSKVKTEEEADSVRFPQPPDHLT
                     DLKEDLDKDECKQEPEHIYETNCHWDGCSKEFDTQDQLVHHINNDHIHGEKKEFVCRWQ
                     DCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCFKAYSRLENLKTHLRSHTGEKPYV
                     CDHEGCNKAFSNASDRAKHQNRTHSNEKPYICKVPGCTKRYTDPSSLRKHVKTVHGPEA
                     HVTKKHRNDIIQKPSLPKENGDNEASAKLSGREHSDSVSRDQEHCLQTRTIKTEDNMMH
                     QSSPGGQSSCSSEPSPYGNTNNIDSGVDVSLAWQGSLGDLFGLEETSPVVDSTVSSWQR
                     SGRPATPETQRIHSAETGTAEEREIKDNERFLLIYEPNATCQNTRLPTISANGFDVIGV
                     PSSVLINPRAIELSMNDVTMMNQLNERRDSTSSTLSSAYTSRRSSGISPYFSSRRSSET
                     SQFGGRLNNSSSADSYDPISTDASRRSSEASQHSGLPNLLNLTPAQHYRLKAKYAAATG
                     GPPPTPLPNMDRIGLRNKLSLMDGADFPLPPFRQLPVPRRCSDGGGNAGLTPMYPHEIP
                     GNNSRRASDPVRRTAGIDDKPLPRFSRFHSMNSMNTLHPPSLSERRNGGLQHYTCSDGG
                     LHRHVYSPRPPSISENVAMEAISCDADVPGGDDDLMLPDDVVQYIRSQNREAPEQNLQT
                     EYSSPARNLQSNTKSFHNNTPEQPRAPGAYLSRNFPALAECLGQTANMQDNNMPVQWNE
                     VSSGTVDVSDLPKQQFAAGNLAVVQQKQNFAQYQSFNQAPMQRAHNIMGQGQESVQRNI
                     SVNGQRFNYLQQRQQQMSQCQIVSSDFIPQQRYSQSQSMLSSRAMQEGQSQISPSCNNM
                     VERPGVHTHAAPSNTLNHQRLAVHGAPTQGFANNFSVNQDGLHPPNAYTVQPQKNGLEP
                     QQNTLGMSGQAFNHGMIQPRPPAAPHPPNRPRNIHPVHHPPYMRSPHPVSELSPGQQTA
                     EATPKRTSENTDPTPKDNNLLYYSGQIHMYEPNGNFGSGIDCTVRQLPTMPSPGANQVT
                     STVDSQGLEHPPVIDFDAMMDDGDHSSLMSGTLSPGLLQSFSQTSSRLTTPRNSLTLPS
                     IPAGINNMAIGDMSSMLTTLAEESKFLNLNRFKAMEQKLISEEDLNEMEQKLISEEDLN
                     EMEQKLISEEDLNEMEQKLISEEDLNEMEQKLISEEDLNEMESLGDLTMEQKLISEEDL
                     NSRPLEPLEL"
     gene            6..4067
                     /gene="Gli2 activator"
                     /label=Gli2 activator
     sig_peptide     3783..4037
                     /gene="Gli2 activator"
     CDS             3786..3815
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3825..3854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3864..3893
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3903..3932
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3942..3971
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4005..4034
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4062..4079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(4084..4218)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4317..4335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4765..4781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4789..4805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4813..4843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4858..4879)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5167..5755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5929..6786)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6787..6891)
                     /label=AmpR promoter
     primer_bind     7365..7381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7703..10787
                     /gene="Xlp-Sla-3000"
                     /regulatory_class="promoter"
     gene            7703..10787
                     /gene="Xlp-Sla-3000"
                     /label=Xlp-Sla-3000
     regulatory      10610..10615
                     /gene="Xlp-Sla-3000"
                     /regulatory_class="TATA_box"
ORIGIN
        1 gatccatggg gggaggggcc ctttccatct ctcctctgtc cgatgccagc attgacctgc
       61 agaccatgat ccgcacctcc cccaactcgc tggttgctta catcaacaac tctcggagca
      121 gctcagccgc cagcgggtcc tacgggcacc tttctgcagg agcaataagc ccggcgttta
      181 gcttccccat ccataaaccc tgtagcctat cagcagctct gagccagcag cgtagcctga
      241 gttcctcatt tggacacacc cctttgctcc acccatcgcc gacgtttgca tcccgccagc
      301 agggagcact cacatcagcc aatccagctc ctcccagcaa caacagctcc gcccctgact
      361 ccgtcctgaa caaggtgagc agtgagtctg cggtgagcag tacagtcaat caggtgatac
      421 acaaacgcag caaagtgaag actgaagaag aggccgattc agtgcgattc ccccagcctc
      481 cggaccacct gactgatctg aaagaggatt tggataagga tgagtgcaag caggagcctg
      541 aacatatata cgagaccaac tgccactggg acggctgcag caaggagttt gatacccagg
      601 atcagctcgt acatcacatc aataatgacc atatccatgg ggagaagaag gagtttgtgt
      661 gtcgctggca ggactgctcc cgggagcaga aaccattcaa ggcgcagtat atgctggtgg
      721 ttcacatgag aaggcacacg ggggagaaac cacataaatg cacctttgaa ggttgtttca
      781 aggcctattc caggctggag aacctgaaga cgcacttgcg ttcacacacc ggggagaagc
      841 catatgtctg tgaccacgag ggatgcaata aggcgttttc caatgcgtct gatcgagcca
      901 aacatcagaa cagaacccat tccaatgaga aaccgtacat atgtaaggtc ccaggctgca
      961 caaaacgtta cacggacccc agctctctca ggaaacacgt taaaactgta catggacctg
     1021 aggcccatgt gaccaagaag caccgcaatg acatcatcca aaaaccttca ctacccaagg
     1081 agaacgggga caatgaagct agtgccaaac tcagcggtag ggagcactcc gacagtgtct
     1141 ctagagacca ggagcactgc ttgcagacca gaaccatcaa aacagaagat aacatgatgc
     1201 atcagtccag ccccggtggg cagtcgtcat gcagcagtga gccttcccca tatggcaaca
     1261 ccaacaacat tgacagtgga gtagatgtga gccttgcatg gcaggggagc ttgggagacc
     1321 tgtttggctt ggaggagacc agcccggtgg tagactccac agtatcatcc tggcagcgca
     1381 gtggtcggcc tgcaactccg gaaacacagc gtatccacag tgcagagact ggaacagctg
     1441 aagaaagaga aattaaagac aatgaaagat tcctgctcat ttatgaaccc aatgccacct
     1501 gccagaacac ccggctcccg accatcagtg caaatggttt cgatgtaata ggtgtcccca
     1561 gcagtgtgct aatcaacccc agagcaatag aactgtccat gaatgatgtg accatgatga
     1621 accaattgaa tgagcgccgt gacagcacca gcagcaccct cagctcagca tacaccagcc
     1681 gcaggtcctc cggcatctct ccttacttct ccagtcgccg ctcaagtgaa acctcacagt
     1741 ttggaggccg cttgaataat tccagctctg cagattctta cgatcccatt tccaccgatg
     1801 cctctaggcg ctctagtgag gccagccagc acagtggact tcctaatctt ctcaacctca
     1861 ctcctgctca gcattacaga ttaaaggcca agtatgctgc agcaactggt ggtccaccac
     1921 ccactcctct tcccaacatg gataggatag gtttgagaaa caagctgtcc ctgatggatg
     1981 gagcagactt tcctttgcct cccttccggc aacttcctgt tccaagacgt tgtagtgatg
     2041 gtggtggaaa tgcaggacta acccccatgt atccccatga aattccgggg aataattcaa
     2101 ggcgggctag tgacccagtc agaagaactg caggtattga tgacaagcca ctcccaaggt
     2161 tcagccgctt tcatagcatg aatagtatga atacattgca ccctccatcc ctatcggaaa
     2221 ggagaaatgg aggcctgcag cactacacct gctctgatgg aggccttcac aggcacgtct
     2281 attctccccg accacccagc attagtgaaa atgttgctat ggaggccata tcttgtgatg
     2341 cagatgtccc tggtggagat gatgacctca tgctcccaga tgatgttgtt cagtacatca
     2401 gatctcagaa cagagaagcc ccagagcaaa acctacagac ggagtacagc agtccagccc
     2461 ggaatctaca aagcaacaca aaatctttcc ataacaatac accagaacaa cccagggccc
     2521 ctggggctta cttgagcagg aactttcctg ctttggctga atgccttggc cagactgcta
     2581 acatgcagga taacaacatg cccgttcagt ggaacgaagt cagttcgggt accgttgatg
     2641 tcagtgactt acccaagcag cagtttgcag caggcaactt agcagttgtt cagcaaaaac
     2701 agaactttgc ccagtatcag agcttcaacc aagcacctat gcagagggct cacaatatta
     2761 tgggtcaggg gcaagagtct gtacagagga atatcagtgt taatggacag cggtttaatt
     2821 atttgcaaca aagacaacag cagatgagcc agtgccaaat tgtcagttct gacttcatcc
     2881 cccaacagag gtacagccag agccagtcaa tgctgagttc aagagcaatg caagaaggcc
     2941 agagccagat ttctccatcc tgtaataaca tggtagagag gcctggggtt cacacacatg
     3001 ctgctccaag caacacactg aatcatcagc gccttgctgt gcacggagca cccacgcagg
     3061 gttttgcaaa taacttctcc gttaaccaag acgggctcca cccacccaac gcctacacag
     3121 tccagcctca gaaaaatgga ttggagccac aacagaacac tcttggaatg tctgggcaag
     3181 cgttcaacca tggaatgatt cagccaagac ccccagctgc cccacacccc cctaacagac
     3241 ccaggaatat acacccggta catcaccctc cttacatgag gagtccgcac cctgtgagtg
     3301 aactgagccc aggccaacaa acagcagagg caacaccaaa acgaaccagt gaaaacactg
     3361 atccaactcc taaagacaat aatttgcttt attattctgg acaaattcac atgtacgagc
     3421 caaatggaaa cttcggttct ggcatagact gcacagtacg gcaactaccc accatgccgt
     3481 ctcctggggc aaatcaggtc acaagcactg tagactccca aggtcttgag cacccccctg
     3541 ttatagattt tgacgccatg atggatgatg gggatcactc gagccttatg tcaggaacct
     3601 tgagccctgg cctcctgcag agcttctccc aaacatcctc ccgccttaca actcccagga
     3661 attccctgac gctcccttcc atccctgctg ggataaacaa catggcaata ggagacatga
     3721 gctccatgct cacaacattg gcggaggaaa gcaagtttct caatctgaat cgatttaaag
     3781 ctatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
     3841 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
     3901 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
     3961 aagaggactt gaatgaaatg gagagcttgg gcgacctcac catggagcaa aagctcattt
     4021 ctgaagagga cttgaattca aggcctctcg agcctctaga actatagtga gtcgtattac
     4081 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4141 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4201 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4261 gaggtgtggg aggtttttta attcgcggcc gccaccgcgg tggagctcca attcgcccta
     4321 tagtgagtcg tattacgcgc gccaacgttc ctcagcccat cacaaagctc tgacctcaat
     4381 cctatagaaa ggaggaatga gccaaaattc acccaactta ttgtgggaag cttgtggaag
     4441 gctactcgaa atgtttgacc caagttaaac aatttaaagg caatgctacc aaatactaat
     4501 tgagtgtatg ttaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg
     4561 aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta
     4621 actgacctta agacagggaa tctttactcg gattaaatgt caggaattgt gaaaaagtga
     4681 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaacttga gtcgatgcag
     4741 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4801 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4861 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4921 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4981 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5581 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6061 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6541 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6601 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6661 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6721 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6781 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6841 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6901 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6961 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     7021 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7081 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7141 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7201 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7261 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7321 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     7381 tgaattcgag cttgcatgtg cagggtaccc tacagttgaa gtcggaagtt tacatacact
     7441 taagttggag tcattaaaac tcgtttttca actacaccac aaatttcttg ttaacaaaca
     7501 atagttttgg caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa
     7561 caattgttta cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc
     7621 agaagtttac atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat
     7681 gatgtcatgg ctttagaagc ttcccagatt aagaagttat gctgtctgac agtaaactcc
     7741 gcttgaacat gttctaacag atttctcagc tcttttcttt catgtttcca tcccaacacc
     7801 tgtcgtatac aaagctaggg gtggtctaac ctcacttctc tattgagctc attataggat
     7861 aaaagaaagg tattgagaga ctggaaatga acgtgaactg gtgggcaggt gtggggccct
     7921 tcagtaagac cactgtgaag taaaggtgct gcacttgaac ttctgcaatg cctcagcctg
     7981 tgaaagaatt tccttactaa tcaaacacaa acactgaaca attcctgctg ctatacaata
     8041 aaagaaagta atatatggaa taggagaatg aattgcagta cgcgctgctg tttttttctg
     8101 taacctcttg tatgttcaat gtacatgcct atttattgta cagcactgca gaacatgtta
     8161 gcacttttta aatacatgat aacaataaat gggtgaatgt gcaccttagt gatgtagccc
     8221 gctcgcttac tctgttctga atgtgcagct tgccactaca tgacttaaag gcttatataa
     8281 caatgcaagt gacacatgta aagtgcatat aaaggcaaat gctgctgctt tttacccaaa
     8341 atgctgattt tttttctcca gaattccagc atgcactgca gtttgatcat gatgtaccag
     8401 tgcaggagca gctacaagtg ggacaagtac cctagaagta atactcctca aggcaggtgt
     8461 taactccaag gttacccatg gtataatctg agcacctgtg cagtgaatgt agctgtagat
     8521 atagcaacat agtaacatag gtcgaaaaaa gatacttttc catgttgaac tcaacctttt
     8581 atgtttaagt gcaacctgtc taactgctag ttgatccaga ggaaggcaaa aaaaaacatc
     8641 tgaagccttg acatccttct tgactctaag atttcaattg gaccagtccc tggatcagtt
     8701 ttgtaccaat acatattttc agtagccctc tatttcctca cttgtaaccc cttcttaaag
     8761 ttatctaaaa tatctgttag tacaatttat tcagggaaaa aataataatc cttgtaggac
     8821 aggaggtaca gtattataga gtaaaatagt gtagcagtga ggataataga ctctgggaag
     8881 ggactgttgc tctgggaaag aaggtatagt agggagagat ggtgcctata gtaacagtgg
     8941 gataatagtc tctgggaagg gagtgtggct gagggatagc aggtatagta gggagagatg
     9001 gtgtctatag taacggtggg ataatagtct ctgggaaggg agcgtggctg agggatagca
     9061 ggtatagtag ggagagatgg tgtctatagt aacagtggga taatagtctc tgggaaggga
     9121 atgtgactgt gggatagcag gtatagtagg gagagatgtg cctatagtaa cagtggatga
     9181 tagtctctgg gaagggagtg ttgctgtggg atagcaggta tagtagggag agatggtgtc
     9241 tatagtaaca gtgggataat agtctctggg aagggagtgt gactgtggga tagcaggtat
     9301 agtagggaga gatggtgcct atagtaacag tgggataata gtctctggga agggagtgtg
     9361 gctgagggat agcaggtata gtagggagag atgtgcctat agtaacagtg acataatagt
     9421 ctctgggaag ggagtgtggt tgtgggatag caggtatagt agggagagat ggtgcctata
     9481 gtaacagtgg ataatagtct ctgggaaggg agtgtggctg agggatagca ggtatagtag
     9541 ggagagatgg tgcctatagt aacagtggat aatagtctct gggaagggag tgtggctgag
     9601 ggatagcagg tatagtaggg agagatggtg cctatagtaa cagtggggat aatagtctct
     9661 gggaagggag tgtcgctgag ggatagcagt atagctgagt agctgagggg ataaccaact
     9721 gggaaggggc atttgttgta agggtattat aggaagagct tttcatctgt actgacaccc
     9781 tatggctgct ggggagtgtc tcatattcaa tttaacaata cactttttaa actggttata
     9841 ttctcaactt tcagggaccc taaaaaaaga tatttttgtg ggagcagtta cttctatggg
     9901 tcccactaac ctaaaaaagg atgtccagct gttcttataa ttttacacca gattccacca
     9961 aaactaagga attccccagg attctgcagg atctcattca caaagctgaa atggttcccg
    10021 ggtcattggc ccaatctaat gaatgaccat ttcttaatgt ctgtgaggcc gagacgctgt
    10081 gtgaaccaga gagcaatgaa agctgcccag aaactgcatt aataataata ataataatat
    10141 agcgtaatta aagtgacctc ttgtataata atatattatc ttgttgtcaa tgtgtgtttt
    10201 accacagccc cattgctata cattgtaggg ctacaggata acagtacagg ggctctttgt
    10261 tgtcaatgtc acccccgggt cggtgcaggg gacaagtttc ccgcctcgtc cattgctaaa
    10321 agcagatccc ctcagcccac tgtctgcccc gggccacatt cccagcaggc gactacatct
    10381 tccactgatg aacactgaca ggactcgggt cttcctctcc gctaaaaact gtccctgtgg
    10441 gaggatctga aacccgagac accatcttac acgattggac gtttcccccc caaaaaaatc
    10501 ctcccctccc aaggaagaaa gctcagtctg tgaggctgct tggaaggaat taggaactta
    10561 ttggggggga ggagcccggt gcctcccatt ctgcacctga ctgctcagct atttacagca
    10621 ctttggaagg agtcttagca ctgccacttg cgctcagcgg tgaatggact gggcgagcac
    10681 aagatgctat aaggatcagc tggcgggcga ggacgaaacc ataatcgctc ctcctgggga
    10741 ccgttacttt aagggaccgt tacttgtgcg tccctcccgc actgaaatct agatctgtag
    10801 gg
//
