AF286076 vector (V010126)

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LOCUS       40924_200        5130 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286076, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Arthos J, Mullins JI.
  TITLE     Multipurpose Eukaryotic Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, WA 98105, USA
REFERENCE   3  (bases 1 to 5130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2000) Microbiology, University of Washington, HSB K-455, 
            Seattle, WA 98105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(24..325)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1185..2008
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     misc_feature    2022..2115
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2030..2092
                     /label=tPA leader
                     /note="tPA leader"
     misc_feature    2109..2220
                     /note="similar to SIV nef; nonfunctional"
     polyA_signal    2241..2465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2486..2808
                     /note="3'end of neomycin resistance; nonfunctional"
     polyA_signal    2809..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2981..2997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3005..3021)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3029..3059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3074..3095)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3383..3971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4145..5002)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5003..5107)
                     /label=AmpR promoter