Basic Vector Information
- Vector Name:
- AF286076
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5130 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Arthos J, Mullins JI.
- Promoter:
- SV40
AF286076 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AF286076 vector Sequence
LOCUS 40924_200 5130 bp DNA circular SYN 17-DEC-2018
DEFINITION Expression vector AF286076, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5130)
AUTHORS Arthos J, Mullins JI.
TITLE Multipurpose Eukaryotic Expression Vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5130)
AUTHORS Mullins JI, Hanley T, Kafsack BFC., Rowen L.
TITLE Direct Submission
JOURNAL Submitted (11-JUL-2000) Microbiology, University of Washington, HSB
K-455, Seattle, WA 98105, USA
REFERENCE 3 (bases 1 to 5130)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5130)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-JUL-2000) Microbiology, University of Washington, HSB K-455,
Seattle, WA 98105, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5130
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(24..325)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 466..845
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 846..1049
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 1185..2008
/label=CMV intron A
/note="human cytomegalovirus intron A (Chapman et al.,
1991)"
misc_feature 2022..2115
/label=multiple cloning site
/note="multiple cloning site"
misc_feature 2030..2092
/label=tPA leader
/note="tPA leader"
misc_feature 2109..2220
/note="similar to SIV nef; nonfunctional"
polyA_signal 2241..2465
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 2486..2808
/note="3'end of neomycin resistance; nonfunctional"
polyA_signal 2809..2943
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2981..2997)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3005..3021)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3029..3059)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3074..3095)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3383..3971)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4145..5002)
/label=AmpR
/note="beta-lactamase"
promoter complement(5003..5107)
/label=AmpR promoter
This page is informational only.