AF286076 vector (V010126)

Basic Vector Information

      • Vector Name:
      • AF286076
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5130 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Arthos J, Mullins JI.

AF286076 vector Vector Map

AF2860765130 bp6001200180024003000360042004800SV40 early promoter/origin derived from pcDNA3.1/Zeo(+)derived from CMVmultiple cloning sitebGH poly(A) signal3'end of neomycin resistance; nonfunctionalSV40 poly(A) signalM13 revsimilar to pUC19

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AF286076 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5130 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286076, complete sequence.
ACCESSION   AF286076
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Arthos J, Mullins JI.
  TITLE     Multipurpose Eukaryotic Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, WA 98105, USA
REFERENCE   3  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Expression vector AF286076"
                     /mol_type="other DNA"
                     /db_xref="taxon:146703"
     regulatory      16..359
                     /regulatory_class="promoter"
                     /note="SV40 early promoter/origin derived from 
                     pcDNA3.1/Zeo(+)"
     regulatory      367..2017
                     /regulatory_class="promoter"
                     /note="derived from CMV"
     regulatory      367..1001
                     /regulatory_class="enhancer"
                     /note="derived from CMV"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1002..1006
                     /regulatory_class="CAAT_signal"
                     /note="derived from CMV"
     regulatory      1035..1040
                     /regulatory_class="TATA_box"
                     /note="derived from CMV"
     intron          1185..2008
                     /note="derived from CMV"
     misc_feature    2022..2115
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2030..2092
                     /label=tPA leader
                     /note="tPA leader"
     misc_feature    2109..2220
                     /note="similar to SIV nef; nonfunctional"
     polyA_signal    2241..2465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      2254..2355
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from bovine growth hormone"
     misc_feature    2486..2808
                     /note="3'end of neomycin resistance; nonfunctional"
     polyA_signal    2809..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2809..2939
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from SV40"
     primer_bind     complement(2981..2997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3001..5130
                     /label=similar to pUC19
                     /note="similar to pUC19"
     protein_bind    3005..3021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3029..3059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3074..3095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3383..3971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4142..5002)
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="AAG45963.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_RNA        4465..5023
                     /product="ColE1 RNA II"
                     /label=replication regulation
                     /note="replication regulation"
     misc_RNA        complement(4469..4575)
                     /product="ColE1 RNA I"
                     /label=replication regulation
                     /note="replication regulation"
     promoter        complement(5003..5107)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5016..5021
                     /label=derived from ColE1
                     /note="derived from ColE1"

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