LOCUS       40924_200        5130 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286076, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Arthos J, Mullins JI.
  TITLE     Multipurpose Eukaryotic Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, WA 98105, USA
REFERENCE   3  (bases 1 to 5130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2000) Microbiology, University of Washington, HSB K-455, 
            Seattle, WA 98105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(24..325)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1185..2008
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     misc_feature    2022..2115
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2030..2092
                     /label=tPA leader
                     /note="tPA leader"
     misc_feature    2109..2220
                     /note="similar to SIV nef; nonfunctional"
     polyA_signal    2241..2465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2486..2808
                     /note="3'end of neomycin resistance; nonfunctional"
     polyA_signal    2809..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2981..2997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3005..3021)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3029..3059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3074..3095)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3383..3971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4145..5002)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5003..5107)
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgcgg ccgctctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
       61 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tgcatggggc
      121 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      181 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      241 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      301 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat taattcccgg
      361 ggatcgatcc ggtcgacaat attggctatt ggccattgca tacgttgtat ctatatcata
      421 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga
      481 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      541 gcgttacata acttacggta aatggcccgc ctcgtgaccg cccaacgacc cccgcccatt
      601 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      721 aagtccggcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      781 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      841 ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg
      901 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      961 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg
     1021 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     1081 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
     1141 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
     1201 gactctatag gcacacccct ttggctctta tgcatgctat actgtttttg gcttggggcc
     1261 tatacacccc cgctccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt
     1321 attgaccatt attgaccact cccctattgg tgacgatact ttccattact aatccataac
     1381 atggctcttt gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac
     1441 tgacacggac tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata
     1501 tacaacaacg ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg
     1561 cgaatctcgg gtacgtgttc cggacatggg ctcttctccg gtagcggcgg agcttccaca
     1621 tccgagccct ggtcccatgc ctccagcggc tcatggtcgc tcggcagctc cttgctccta
     1681 acagtggagg ccagacttag gcacagcaca atgcccacca ccaccagtgt gccgcacaag
     1741 gccgtggcgg tagggtatgt gtctgaaaat gagctcggag attgggctcg caccgtgacg
     1801 cagatggaag acttaaggca gcggcagaag aagatgcagg cagctgagtt gttgtattct
     1861 gataagagtc agaggtaact cccgttgcgg tgctgttaac ggtggagggc agtgtagtct
     1921 gagcagtact cgttgctgcc gcgcgcgcca ccagacataa tagctgacag actaacagac
     1981 tgttcctttc catgggtctt ttctgcagtc accgtccaag cttgcaatca tggatgcaat
     2041 gaagagaggg ctctgctgtg tgctgctgct gtgtggagca gtcttcgttt cggctagccc
     2101 gggacgcgtg gatcctcgca atccctagga ggattaggca agggcttgag ctcacgctct
     2161 tgtgagggac agaaatacaa tcaggggcag tatatgaata ctccatggag aaacccagat
     2221 ctacgtatga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2281 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2341 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2401 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     2461 tatggaacca gctggggctc gacaggaccg ctatcaggac atagcgttgg ctacccgtga
     2521 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     2581 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     2641 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     2701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     2761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     2821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2941 atcgcggccg cgatcccgtc gagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     3001 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3061 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3121 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3181 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3241 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3301 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3361 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3541 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     3601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3721 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3781 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3841 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3901 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3961 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4021 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4081 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4141 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4201 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4261 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4321 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4381 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4441 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4501 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4561 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4621 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4681 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4741 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4801 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4861 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4921 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4981 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5041 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5101 ttccgcgcac atttccccga aaagtgccac
//
