AbVec-hIgG1 vector (V010129)

Basic Vector Information

      • Vector Name:
      • AbVec-hIgG1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5746 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, Charpilienne A, Poncet D, Wilson PC, Sollid LM.

AbVec-hIgG1 vector Vector Map

AbVec-hIgG15746 bp60012001800240030003600420048005400CMVSP6 promotermurine IgG1 signal peptide; derived from DEC205/immunoglobluin gamma fusion protein in GenBank Accession Number DQ407610heavy chain variable gene cloning site; flanked by AgeI and SalI sitesIgG1vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)SV40 poly(A) signalSV40 promoterM13 fwdf1 oriAmpR promoterAmp_Resorilac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AbVec-hIgG1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5746 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AbVec-hIgG1, complete sequence.
ACCESSION   FJ475055
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, 
            Charpilienne A, Poncet D, Wilson PC, Sollid LM.
  TITLE     Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies 
            from Small-Intestinal Mucosa
  JOURNAL   J. Immunol. 185 (9), 5377-5383 (2010)
  PUBMED    20935207
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Antibody variable gene expression vector for human IgG1 heavy chain
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2008) Medicine/Rheumatology, The University of 
            Chicago, BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 
            60637, USA
REFERENCE   4  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Cloning vector AbVec-hIgG1"
                     /mol_type="other DNA"
                     /note="expression cloning vector for human antibody 
                     variable genes"
                     /db_xref="taxon:583342"
     regulatory      1..618
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    929..973
                     /note="murine IgG1 signal peptide; derived from 
                     DEC205/immunoglobluin gamma fusion protein in GenBank 
                     Accession Number DQ407610"
     misc_feature    974..991
                     /note="heavy chain variable gene cloning site; flanked by 
                     AgeI and SalI sites"
     CDS             992..1984
                     /codon_start=1
                     /gene="IgG1"
                     /product="IgG1"
                     /label=IgG1
                     /note="Cgamma-1; derived from Homo sapiens"
                     /protein_id="ACK87036.1"
                     /translation="RSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD
                     KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
                     GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
                     KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
                     DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
     gene            992..1984
                     /gene="IgG1"
                     /label=IgG1
     regulatory      1991..2000
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2004..2138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2211..2568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2419..2554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2588..2604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2817..3272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3604..3708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3709..4569
                     /codon_start=1
                     /gene="Amp_Res"
                     /product="beta-lactamase"
                     /label=Amp_Res
                     /note="confers resistance to ampicillin"
                     /protein_id="ACK87037.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3709..4569
                     /gene="Amp_Res"
                     /label=Amp_Res
     rep_origin      4740..5328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5652..5682
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5714..5730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

This page is informational only.