LOCUS       40924_190        5746 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AbVec-hIgG1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, 
            Charpilienne A, Poncet D, Wilson PC, Sollid LM.
  TITLE     Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies 
            from Small-Intestinal Mucosa
  JOURNAL   J. Immunol. 185 (9), 5377-5383 (2010)
  PUBMED    20935207
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Antibody variable gene expression vector for human IgG1 heavy chain
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2008) Medicine/Rheumatology, The University of 
            Chicago, BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 
            60637, USA
REFERENCE   4  (bases 1 to 5746)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2010"; volume: "185"; issue: "9"; pages: 
            "5377-5383"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2008) Medicine/Rheumatology, The University of Chicago, 
            BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             929..985
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     CDS             995..1981
                     /label=hlgG1-CH
                     /note="Human IgG1 heavy chain constant region"
     regulatory      1991..2000
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2004..2138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2211..2568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2588..2604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2817..3272
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3604..3708
                     /label=AmpR promoter
     CDS             3709..4566
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4740..5328
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5616..5637
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5652..5682
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5706
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5714..5730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt
      961 agcaactgca accggtgtac actcgagcgt acggtcgacc aagggcccat cggtcttccc
     1021 cctggcaccc tcctccaaga gcacctctgg gggcacagcg gccctgggct gcctggtcaa
     1081 ggactacttc cccgaacctg tgacggtctc gtggaactca ggcgccctga ccagcggcgt
     1141 gcacaccttc ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac
     1201 cgtgccctcc agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag
     1261 caacaccaag gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc
     1321 accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc
     1381 caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag
     1441 ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc
     1501 caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac
     1561 cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc
     1621 cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca
     1681 ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg
     1741 cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc
     1801 ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta
     1861 cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt
     1921 gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa
     1981 atgaagcttg gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa
     2041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2101 tgtccaaact catcaatgta tcttatcatg tctggatcga tcgggaatta attcggcgca
     2161 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     2221 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     2281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     2341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     2401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2521 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca
     2581 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2641 ttaatcgcct tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca
     2701 ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga atggcgcctg atgcggtatt
     2761 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     2821 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2881 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2941 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3001 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     3061 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3121 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     3181 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3241 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     3301 tgatgccgca tagttaagcc aactccgcta tcgctacgtg actgggtcat ggctgcgccc
     3361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3481 ccgaaacgcg cgaggcagta ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     3541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgatgacgcc
     3961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc agcagcaatg
     4261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4861 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5101 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     5161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccagctggca cgacaggttt
     5581 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct cactcattag
     5641 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5701 taacaatttc acacaggaaa cagctatgac catgattacg aattaa
//
