pLXSN-HXY-TCR vector (V013994)

MSDS

Basic Vector Information

Vector Name:: pLXSN-HXY-TCR

Antibiotic Resistance:: Ampicillin

Length:: 7676 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells, Retrovirus

Selection Marker:: Neo/G418

Promoter:: SV40

Growth Strain(s):: Stbl3

Growth Temperature:: 37℃

pLXSN-HXY-TCR vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLXSN-HXY-TCR vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16370        7676 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..589
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1482..1826
                     /gene="TRBV11-2"
                     /label=TRBV11-2
                     /note="T cell receptor beta variable 11-2 from Homo
                     sapiens. Accession#: A0A584"
     CDS             2421..2477
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             2478..2813
                     /gene="TRAV17"
                     /label=TRAV17
                     /note="T cell receptor alpha variable 17 from Homo sapiens.
                     Accession#: A0A0B4J275"
     CDS             2877..3284
                     /gene="Trac"
                     /label=Trac
                     /note="T-cell receptor alpha chain constant from Mus
                     musculus. Accession#: P01849"
     promoter        3305..3634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3694..4485
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     LTR             4548..5141
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    5352..5492
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5678..6266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6440..7297)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7298..7402)
                     /label=AmpR promoter

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