LOCUS       V013994                 7676 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013994
VERSION     V013994
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..589
                     /label="5' LTR"
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    652..851
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1052..1468
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1482..1826
                     /gene="TRBV11-2"
                     /label="T cell receptor beta variable 11-2"
                     /note="T cell receptor beta variable 11-2 from Homo
                     sapiens. Accession#: A0A584"
     CDS             2421..2477
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             2478..2813
                     /gene="TRAV17"
                     /label="T cell receptor alpha variable 17"
                     /note="T cell receptor alpha variable 17 from Homo sapiens.
                     Accession#: A0A0B4J275"
     CDS             2877..3284
                     /gene="Trac"
                     /label="T-cell receptor alpha chain constant"
                     /note="T-cell receptor alpha chain constant from Mus
                     musculus. Accession#: P01849"
     promoter        3305..3634
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3694..4485
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             4548..5141
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    5352..5492
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5678..6266)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6440..7297)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7298..7402)
                     /label="AmpR promoter"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattcgccac catgggcacc cgcctgctgt
     1501 gctgggccgc cctgtgcctg ctgggcgccg agctgaccga agctggagtt gcccagtctc
     1561 ccagatataa gattatagag aaaaggcaga gtgtggcttt ttggtgcaat cctatatctg
     1621 gccatgctac cctttactgg taccagcaga tcctgggaca gggcccaaag cttctgattc
     1681 agtttcagaa taacggtgta gtggatgatt cacagttgcc taaggatcga ttttctgcag
     1741 agaggctcaa aggagtagac tccactctca agatccagcc tgcaaagctt gaggactcgg
     1801 ccgtgtatct ctgtgccagc agcttggggg atacctacga gcagtacttc gggccgggca
     1861 ccaggctcac ggtcacagag gacctgcgca atgtgacccc ccctaaggtg tccctgtttg
     1921 agccttctaa ggccgagatc gccaacaagc agaaggccac cctggtgtgc ctggcaaggg
     1981 gcttctttcc agatcacgtg gagctgtcct ggtgggtgaa tggcaaggag gtgcactctg
     2041 gcgtgtgcac cgacccacag gcctacaagg agagcaacta ctcctattgt ctgtctagcc
     2101 ggctgagagt gtccgccaca ttctggcaca acccaaggaa tcacttccgc tgccaggtgc
     2161 agtttcacgg cctgtctgag gaggataagt ggccagaggg aagcccaaag ccagtgaccc
     2221 agaatatctc cgccgaggca tggggaaggg cagactgtgg aatcacctcc gcctcttatc
     2281 agcagggcgt gctgagcgcc acaatcctgt acgagatcct gctgggcaag gccaccctgt
     2341 atgccgtgct ggtgtctaca ctggtggtca tggctatggt gaagcgcaag aatagcaggg
     2401 caaagcgcag cggatccgga gcaaccaact tctccctgct gaagcaggca ggcgatgtgg
     2461 aggagaaccc tggaccaatg gagaccctgc tgggcgtgag cctggtgatc ctgtggctgc
     2521 agctggcccg cgtgaacagt caacagggag aagaggatcc tcaggccttg agcatccagg
     2581 agggtgaaaa tgccaccatg aactgcagtt acaaaactag tataaacaat ttacagtggt
     2641 atagacaaaa ttcaggtaga ggccttgtcc acctaatttt aatacgttca aatgaaagag
     2701 agaaacacag tggaagatta agagtcacgc ttgacacttc caagaaaagc agttccttgt
     2761 tgatcacggc ttcccgggca gcagacactg cttcttactt ctgtgctacg gacggacaaa
     2821 ctggggcaaa caacctcttc tttgggactg gaacgagact caccgttatt cccaatatcc
     2881 agaatcccga gcctgccgtg taccagctga aggaccctcg ctcccaggat tctaccctgt
     2941 gcctgttcac agactttgat agccagatca acgtgcccaa gacaatggag tccggcacct
     3001 tcatcacaga caagtgcgtg ctggatatga aggctatgga ctccaagtct aacggcgcca
     3061 tcgcctggtc taatcagacc agcttcacat gccaggatat ctttaaggag accaacgcca
     3121 catatccatc tagcgacgtg ccctgtgatg ccaccctgac agagaagagc ttcgagaccg
     3181 acatgaacct gaattttcag aatctgctgg tcatcgtgct gaggatcctg ctgctgaagg
     3241 tggccggctt taacctgctg atgacactgc gcctgtggtc ctcttgagga tccggctgtg
     3301 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3361 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3601 aggaggcttt tttggaggcc taggcttttg caaaaagctt gggctgcagg tcgaggcgga
     3661 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3721 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3781 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3841 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     3901 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3961 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4021 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4081 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4141 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4201 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     4261 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4321 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4381 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4441 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4501 ggttcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     4561 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     4621 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     4681 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     4741 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     4801 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     4861 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     4921 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     4981 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     5041 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     5101 cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc
     5161 gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg
     5221 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     5281 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     5341 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     5401 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     5461 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6721 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7021 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7501 aagaattgct agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt
     7561 cctccaaatg tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc
     7621 taccctattc cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct
//
