pPIC9k-CBDAS(Cannabis)-6×His vector (V013984)

MSDS

Basic Vector Information

Vector Name:: pPIC9k-CBDAS(Cannabis)-6×His

Antibiotic Resistance:: Ampicillin

Length:: 10851 bp

Type:: Protein expression

Replication origin:: ori

Host:: Yeast

Selection Marker:: HIS4,Neo/G418

Promoter:: AOX1

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pPIC9k-CBDAS(Cannabis)-6×His vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPIC9k-CBDAS(Cannabis)-6×His vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V013984                10851 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013984
VERSION     V013984
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             1147..2778
                     /gene="CBDAS"
                     /label="Cannabidiolic acid synthase"
                     /note="Cannabidiolic acid synthase from Cannabis sativa.
                     Accession#: A6P6V9"
     CDS             2803..2820
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2896..3142
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(6506..7318)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7697..8453
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    8596..8736
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8922..9510)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9684..10541)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10542..10646)
                     /label="AmpR promoter"

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