LOCUS       V013984                10851 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013984
VERSION     V013984
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             1147..2778
                     /gene="CBDAS"
                     /label="Cannabidiolic acid synthase"
                     /note="Cannabidiolic acid synthase from Cannabis sativa.
                     Accession#: A6P6V9"
     CDS             2803..2820
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2896..3142
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(6506..7318)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7697..8453
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    8596..8736
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8922..9510)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9684..10541)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10542..10646)
                     /label="AmpR promoter"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataatga agtgctcaac attctccttt tggtttgttt gcaagataat atttttcttt
     1201 ttctcattca atatccaaac ttccattgct aatcctcgag aaaacttcct taaatgcttc
     1261 tcgcaatata ttcccaataa tgcaacaaat ctaaaactcg tatacactca aaacaaccca
     1321 ttgtatatgt ctgtcctaaa ttcgacaata cacaatctta gattcacctc tgacacaacc
     1381 ccaaaaccac ttgttatcgt cactccttca catgtctctc atatccaagg cactattcta
     1441 tgctccaaga aagttggctt gcagattcga actcgaagtg gtggtcatga ttctgagggc
     1501 atgtcctaca tatctcaagt cccatttgtt atagtagact tgagaaacat gcgttcaatc
     1561 aaaatagatg ttcatagcca aactgcatgg gttgaagccg gagctaccct tggagaagtt
     1621 tattattggg ttaatgagaa aaatgagaat cttagtttgg cggctgggta ttgccctact
     1681 gtttgcgcag gtggacactt tggtggagga ggctatggac cattgatgag aaactatggc
     1741 ctcgcggctg ataatatcat tgatgcacac ttagtcaacg ttcatggaaa agtgctagat
     1801 cgaaaatcta tgggggaaga tctcttttgg gctttacgtg gtggtggagc agaaagcttc
     1861 ggaatcattg tagcatggaa aattagactg gttgctgtcc caaagtctac tatgtttagt
     1921 gttaaaaaga tcatggagat acatgagctt gtcaagttag ttaacaaatg gcaaaatatt
     1981 gcttacaagt atgacaaaga tttattactc atgactcact tcataactag gaacattaca
     2041 gataatcaag ggaagaataa gacagcaata cacacttact tctcttcagt tttccttggt
     2101 ggagtggata gtctagtcga cttgatgaac aagagttttc ctgagttggg tattaaaaaa
     2161 acggattgca gacaattgag ctggattgat actatcatct tctatagtgg tgttgtaaat
     2221 tacgacactg ataattttaa caaggaaatt ttgcttgata gatccgctgg gcagaacggt
     2281 gctttcaaga ttaagttaga ctacgttaag aaaccaattc cagaatctgt atttgtccaa
     2341 attttggaaa aattatatga agaagatata ggagctggga tgtatgcgtt gtacccttac
     2401 ggtggtataa tggatgagat ttcagaatca gcaattccat tccctcatcg agctggaatc
     2461 ttgtatgagt tatggtacat atgtagttgg gagaagcaag aagataacga aaagcatcta
     2521 aactggatta gaaatattta taacttcatg actccttatg tgtccaaaaa tccaagattg
     2581 gcatatctca attatagaga ccttgatata ggaataaatg atcccaagaa tccaaataat
     2641 tacacacaag cacgtatttg gggtgagaag tattttggta aaaattttga caggctagta
     2701 aaagtgaaaa ccctggttga tcccaataac ttttttagaa acgaacaaag catcccacct
     2761 cttccacggc atcgtcatga attccctagg gcggccgcga atcatcatca ccatcaccat
     2821 taattcgcct tagacatgac tgttcctcag ttcaagttgg gcacttacga gaagaccggt
     2881 cttgctagat tctaatcaag aggatgtcag aatgccattt gcctgagaga tgcaggcttc
     2941 atttttgata cttttttatt tgtaacctat atagtatagg attttttttg tcattttgtt
     3001 tcttctcgta cgagcttgct cctgatcagc ctatctcgca gctgatgaat atcttgtggt
     3061 aggggtttgg gaaaatcatt cgagtttgat gtttttcttg gtatttccca ctcctcttca
     3121 gagtacagaa gattaagtga gaagttcgtt tgtgcaagct tatcgataag ctttaatgcg
     3181 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     3241 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     3301 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     3361 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     3421 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     3481 actacgcgat catggcgacc acacccgtcc tgtggatcta tcgaatctaa atgtaagtta
     3541 aaatctctaa ataattaaat aagtcccagt ttctccatac gaaccttaac agcattgcgg
     3601 tgagcatcta gaccttcaac agcagccaga tccatcactg cttggccaat atgtttcagt
     3661 ccctcaggag ttacgtcttg tgaagtgatg aacttctgga aggttgcagt gttaactccg
     3721 ctgtattgac gggcatatcc gtacgttggc aaagtgtggt tggtaccgga ggagtaatct
     3781 ccacaactct ctggagagta ggcaccaaca aacacagatc cagcgtgttg tacttgatca
     3841 acataagaag aagcattctc gatttgcagg atcaagtgtt caggagcgta ctgattggac
     3901 atttccaaag cctgctcgta ggttgcaacc gatagggttg tagagtgtgc aatacacttg
     3961 cgtacaattt caacccttgg caactgcaca gcttggttgt gaacagcatc ttcaattctg
     4021 gcaagctcct tgtctgtcat atcgacagcc aacagaatca cctgggaatc aataccatgt
     4081 tcagcttgag acagaaggtc tgaggcaacg aaatctggat cagcgtattt atcagcaata
     4141 actagaactt cagaaggccc agcaggcatg tcaatactac acagggctga tgtgtcattt
     4201 tgaaccatca tcttggcagc agtaacgaac tggtttcctg gaccaaatat tttgtcacac
     4261 ttaggaacag tttctgttcc gtaagccata gcagctactg cctgggcgcc tcctgctagc
     4321 acgatacact tagcaccaac cttgtgggca acgtagatga cttctggggt aagggtacca
     4381 tccttcttag gtggagatgc aaaaacaatt tctttgcaac cagcaacttt ggcaggaaca
     4441 cccagcatca gggaagtgga aggcagaatt gcggttccac caggaatata gaggccaact
     4501 ttctcaatag gtcttgcaaa acgagagcag actacaccag ggcaagtctc aacttgcaac
     4561 gtctccgtta gttgagcttc atggaatttc ctgacgttat ctatagagag atcaatggct
     4621 ctcttaacgt tatctggcaa ttgcataagt tcctctggga aaggagcttc taacacaggt
     4681 gtcttcaaag cgactccatc aaacttggca gttagttcta aaagggcttt gtcaccattt
     4741 tgacgaacat tgtcgacaat tggtttgact aattccataa tctgttccgt tttctggata
     4801 ggacgacgaa gggcatcttc aatttcttgt gaggaggcct tagaaacgtc aattttgcac
     4861 aattcaatac gaccttcaga agggacttct ttaggtttgg attcttcttt aggttgttcc
     4921 ttggtgtatc ctggcttggc atctcctttc cttctagtga cctttaggga cttcatatcc
     4981 aggtttctct ccacctcgtc caacgtcaca ccgtacttgg cacatctaac taatgcaaaa
     5041 taaaataagt cagcacattc ccaggctata tcttccttgg atttagcttc tgcaagttca
     5101 tcagcttcct ccctaatttt agcgttcaac aaaacttcgt cgtcaaataa ccgtttggta
     5161 taagaacctt ctggagcatt gctcttacga tcccacaagg tggcttccat ggctctaaga
     5221 ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac agaaaccaac acctgtttgt
     5281 tcaaccacaa atttcaagca gtctccatca caatccaatt cgatacccag caacttttga
     5341 gttgctccag atgtagcacc tttataccac aaaccgtgac gacgagattg gtagactcca
     5401 gtttgtgtcc ttatagcctc cggaatagac tttttggacg agtacaccag gcccaacgag
     5461 taattagaag agtcagccac caaagtagtg aatagaccat cggggcggtc agtagtcaaa
     5521 gacgccaaca aaatttcact gacagggaac tttttgacat cttcagaaag ttcgtattca
     5581 gtagtcaatt gccgagcatc aataatgggg attataccag aagcaacagt ggaagtcaca
     5641 tctaccaact ttgcggtctc agaaaaagca taaacagttc tactaccgcc attagtgaaa
     5701 cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga tactagcatt agcgggcaag
     5761 gatgcaactt tatcaaccag ggtcctatag ataaccctag cgcctgggat catcctttgg
     5821 acaactcttt ctgccaaatc taggtccaaa atcacttcat tgataccatt attgtacaac
     5881 ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg taacggatga ctcaacttgc
     5941 acattaactt gaagctcagt cgattgagtg aacttgatca ggttgtgcag ctggtcagca
     6001 gcatagggaa acacggcttt tcctaccaaa ctcaaggaat tatcaaactc tgcaacactt
     6061 gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg gacagtgagt gtagtcttga
     6121 gaaattctga agccgtattt ttattatcag tgagtcagtc atcaggagat cctctacgcc
     6181 ggacgcatcg tggccgacct gcaggggggg ggggggcgct gaggtctgcc tcgtgaagaa
     6241 ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag
     6301 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt
     6361 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa
     6421 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt
     6481 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     6541 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     6601 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     6661 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     6721 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
     6781 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     6841 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     6901 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     6961 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     7021 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     7081 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     7141 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     7201 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     7261 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     7321 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     7381 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttcc cccccccccc
     7441 tgcaggtcgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     7501 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     7561 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     7621 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     7681 cgcataaggg agagcgtcga gtatctatga ttggaagtat gggaatggtg atacccgcat
     7741 tcttcagtgt cttgaggtct cctatcagat tatgcccaac taaagcaacc ggaggaggag
     7801 atttcatggt aaatttctct gacttttggt catcagtaga ctcgaactgt gagactatct
     7861 cggttatgac agcagaaatg tccttcttgg agacagtaaa tgaagtccca ccaataaaga
     7921 aatccttgtt atcaggaaca aacttcttgt ttcgaacttt ttcggtgcct tgaactataa
     7981 aatgtagagt ggatatgtcg ggtaggaatg gagcgggcaa atgcttacct tctggacctt
     8041 caagaggtat gtagggtttg tagatactga tgccaacttc agtgacaacg ttgctatttc
     8101 gttcaaacca ttccgaatcc agagaaatca aagttgtttg tctactattg atccaagcca
     8161 gtgcggtctt gaaactgaca atagtgtgct cgtgttttga ggtcatcttt gtatgaataa
     8221 atctagtctt tgatctaaat aatcttgacg agccaaggcg ataaataccc aaatctaaaa
     8281 ctcttttaaa acgttaaaag gacaagtatg tctgcctgta ttaaacccca aatcagctcg
     8341 tagtctgatc ctcatcaact tgaggggcac tatcttgttt tagagaaatt tgcggagatg
     8401 cgatatcgag aaaaaggtac gctgatttta aacgtgaaat ttatctcaag atctctgcct
     8461 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8521 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8581 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     8641 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     8701 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     8761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     9121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9361 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9961 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
    10021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
    10321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10681 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    10741 cttcaagaat taattctcat gtttgacagc ttatcatcga taagctgact catgttggta
    10801 ttgtgaaata gacgcagatc gggaacactg aaaaataaca gttattattc g
//
