Basic Vector Information
- Vector Name:
- pPD9567-mCherry
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4711 bp
- Type:
- Luciferase reporter
- Replication origin:
- ori
- Host:
- Insect cells
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pPD9567-mCherry vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPD9567-mCherry vector Sequence
LOCUS 62056_18375 4711 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4711) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4711 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 78..752 /codon_start=1 /label=DsRed2 /note="improved tetrameric variant of DsRed fluorescent protein" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" promoter 2456..2560 /label=AmpR promoter CDS 2561..3418 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3592..4180 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4468..4489 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4504..4534 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4542..4558 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4566..4582 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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