LOCUS       62056_18375        4711 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             78..752
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     promoter        2456..2560
                     /label=AmpR promoter
     CDS             2561..3418
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3592..4180
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4468..4489
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4504..4534
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4542..4558
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4566..4582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gctcgagatg gcttcttctg aaaatgttat tactgaattc atgagattca
      121 aagttagaat ggaaggaact gttaatggac atgaattcga aattgaagga gaaggagaag
      181 gaagaccata tgaaggacat aatactgtta aacttaaagt tactaaagga ggaccacttc
      241 cattcgcttg ggatattctt tctccacaat tccaatatgg atctaaagtt tatgttaaac
      301 atccagctga tattccagat tataaaaaac tttctttccc agaaggattc aaatgggaaa
      361 gagttatgaa tttcgaagat ggaggagttg ctactgttac tcaagattct tctcttcaag
      421 atggatgttt catttataaa gttaaattca ttggagttaa tttcccatct gatggaccag
      481 ttatgcaaaa aaaaactatg ggatgggaag cttctactga aagactttat ccaagagatg
      541 gagttcttaa aggagaaact cataaagctc ttaaacttaa agatggagga cattatcttg
      601 ttgaattcaa atctatttat atggctaaaa aaccagttca acttccagga tattattatg
      661 ttgatgctaa acttgatatt acttctcata atgaagatta tactattgtt gaacaatatg
      721 aaagaactga aggaagacat catcttttcc tttaagcggc cgccatacat atttaaattt
      781 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      841 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      901 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
      961 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     1021 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1081 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1141 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1201 catggcatgg atgaactata caaatagcat tcgtagaatt ccaactgagc gccggtcgct
     1261 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     1321 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     1381 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     1441 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     1501 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     1561 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     1621 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     1681 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     1741 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     1801 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     1861 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     1921 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     1981 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     2041 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     2101 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2161 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2221 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2281 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2341 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     2401 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2461 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2521 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2581 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2641 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2701 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2761 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2821 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2881 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2941 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3001 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3061 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3121 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3181 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3241 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3301 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3361 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3661 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3721 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3781 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3841 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3901 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3961 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     4021 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4081 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4141 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4201 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4261 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4321 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4381 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     4441 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     4501 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     4561 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     4621 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     4681 gatggatacg ctaacaactt ggaaatgaaa t
//
