pMD19-Virb8(Bruc)-5UTR(361-1000bp)-Gen-Virb8(Bruc)-3UTR(1721-2505bp) vector (V013812)

MSDS

Basic Vector Information

Vector Name:: pMD19-Virb8(Bruc)-5UTR(361-1000bp)-Gen-Virb8(Bruc)-3UTR(1721-2505bp)

Antibiotic Resistance:: Ampicillin

Length:: 4752 bp

Type:: Gene template

Replication origin:: ori

Host:: E. coli

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pMD19-Virb8(Bruc)-5UTR(361-1000bp)-Gen-Virb8(Bruc)-3UTR(1721-2505bp) vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMD19-Virb8(Bruc)-5UTR(361-1000bp)-Gen-Virb8(Bruc)-3UTR(1721-2505bp) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16660        4752 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..577
                     /label=AmpR promoter
     CDS             578..1435
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1609..2197
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2485..2506
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2521..2551
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2559..2575
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2583..2599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3410..3940
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(4735..4751)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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