LOCUS       62056_16660        4752 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..577
                     /label=AmpR promoter
     CDS             578..1435
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1609..2197
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2485..2506
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2521..2551
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2559..2575
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2583..2599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3410..3940
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(4735..4751)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
       61 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      121 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      181 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      241 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
      301 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
      361 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      421 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      481 ccccatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2221 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2281 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2341 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2401 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2461 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2521 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2581 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagt tcctgcctct
     2641 cactgaccgc tgtaacgatt gtcatgccgt atatgatgta caaggtctat gaatacggtg
     2701 gcatcctggg cagcagcata tcggcggcca cgatctccct cggctcactt gcagtcaata
     2761 ccgcgaaatc tggtggtggc gctatgacga gcatattcag cggatcatcc ggcggcgggg
     2821 gttccggctc ggcgaaggcg ggcggggaaa gcagttattc ggctggtggc aacgccatgt
     2881 ggagcccggc tttccggcaa cacgtgctcg gccagttcaa cagggattag ggccttacac
     2941 gcggcggctt cgccgcagaa tcccgatctt cgcggctacc catgtctggc acatcatggg
     3001 cgaccttttt gcaacgctaa agcgaaaatg aggcaccccg gttttggtcg tactacggcc
     3061 gggaatgctt catccgcacc gcaggtaatc ataatgaaaa aggtaatcct tgcttttgtc
     3121 gccacggcct tccttgccgg ttgcactaca acggggccgg ccgtggtgcc ggttctcgat
     3181 ggcaaaccgc gggttcccgt caacaaaagc gttccggcta aaccgcccct ggctcagcct
     3241 aaccccgttg acacttacga ggactaatcc gaacgcagcg gtggtaacgg cgcagtggcg
     3301 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
     3361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     3421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
     3481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
     3541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
     3601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
     3661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aggtttgagc agccgcgtag
     3721 tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac
     3781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
     3841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
     3901 agaagtgatg cactttgata tcgacccaag taccgccacc taaaaagaat tcctgcttgc
     3961 gtgcatactg atcacgctgg cctcgccctc ctgggcaacc aaaatcccgt ccggctcaaa
     4021 atacgacagc cgaattcagt atgtcgatta caattccggc gatgtcgtgc tggttcgggc
     4081 cctgcccggc gttggcgcac gcatcgtgtt tgcgccgggc gagaatattg aggatgtcgc
     4141 ctcaggattt acgcaaggct gggagttcaa ggcgagccac aacatcctgt atctcaaggc
     4201 ccgctccatg acgctctccc actcgaacca atcgatcgac atggccccgg aaccgggcaa
     4261 gtgggacaca aacctgatgg tcacgacaga tcagcgcatg tacgatttcg atttgcgctt
     4321 gatgcccggc cgcaacaatc agcgtgttgc ctatcgtgtt cagttccgct acccggcggc
     4381 agcggcagcg gcggcagtag cagccgcgca aaagcgagtg gtacaggccc gcatgaatgc
     4441 cagaccgtca ccggtaaact ggaactacac gatgcaggtc ggcactaatt cggccagcat
     4501 cgcgcccact cttgcctatg acgatgggcg gttcacatat ttgcgctttc cgaacaacag
     4561 agactttccg gccgctttcc ttgtcgccga agacaagtcg gaaagcatcg tgaacagcca
     4621 tatagacccg tcagcgccgg acattctggt cctgcatcgg gtcgcaaagc aaatggtgct
     4681 gcgcctcggt aacaaggtga ttggcatcta caacgaatcc ttcaatccga attcactggc
     4741 cgtcgtttta ca
//
