Basic Vector Information
- Vector Name:
- pMLAV-EF1a-MCS-IRES-EGFP-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7819 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Selection Marker:
- Puro
- Promoter:
- EF1a
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pMLAV-EF1a-MCS-IRES-EGFP-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMLAV-EF1a-MCS-IRES-EGFP-Puro vector Sequence
LOCUS 62056_17140 7819 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7819)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7819
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(60..435)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
promoter complement(620..724)
/label=AmpR promoter
repeat_region 838..940
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 1028..1178
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
primer_bind 1212..1228
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 1447..1658
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
misc_feature 1916..2499
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2515..3231
/label=EGFP
/note="enhanced GFP"
polyA_signal complement(3857..3991)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS 4559..4978
/gene="IX"
/label=IX
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(5044..6378)
/gene="IVa2"
/label=IVa2
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
rep_origin complement(7231..7819)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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