LOCUS       V013796                 7819 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013796
VERSION     V013796
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(60..435)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(620..724)
                     /label="AmpR promoter"
     repeat_region   838..940
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     1028..1178
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     1212..1228
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1447..1658
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     misc_feature    1916..2499
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2515..3231
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    complement(3857..3991)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             4559..4978
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(5044..6378)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(7231..7819)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tccatccagt ctataattgt gccggaagct agagtagtag tccccagtta tagttgcgca
       61 acgttgtgcc atgctgcagg catcgtgtgt cacgctcgtc gtttggtatg gcttcattca
      121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      361 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
      421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      721 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
      781 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaattaatt aagcaagcat
      841 catcaataat ataccttatt ttggattgaa gccaatatga taatgagggg gtggagtttg
      901 tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag tagtgtggcg gaagtgtgat
      961 gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac gtttttggtg
     1021 tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat gttgtagtaa
     1081 atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat aagaggaagt
     1141 gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg agatccggta
     1201 ccgtttaaac tcgaggtcga cggtatcgat gcctccccgt caccaccccc cccaacccgc
     1261 cccgaccgga gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca
     1321 gggaccgtcg ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct
     1381 cacccgcccg ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc
     1441 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1501 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1561 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     1621 acgttctttt tcgcaacggg tttgccgcca gaacacaggt acacatattg accaaatcag
     1681 ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt
     1741 atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat
     1801 catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc
     1861 cgattagttc tcgaggatcc gactgaagtc gctagcacga ctgcataggg ttacccccct
     1921 ctccctcccc cccccctaac gttactggcc gaagtcgctt ggaataaggc cggtgtgcgt
     1981 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2041 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     2101 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     2161 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     2221 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     2281 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     2341 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     2401 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     2461 ggttttcctt tgaaaaacac gatgataatg gccacaaccg aattctgaca caccatggtg
     2521 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2581 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2641 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2701 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2761 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2821 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2881 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2941 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3001 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3061 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3121 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3181 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggccaccatg
     3241 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     3301 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     3361 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     3421 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     3481 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     3541 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     3601 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     3661 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     3721 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     3781 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg
     3841 gccgccaccg cggggagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     3901 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3961 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4021 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4081 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4141 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4201 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact
     4261 ctagtccccg cggtggcaga tctggaaggt gctgaggtac gatgagaccc gcaccaggtg
     4321 cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc tggatgtgac
     4381 cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt ttggctctag
     4441 cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa
     4501 tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat
     4561 gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc gcatgccccc
     4621 atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc
     4681 cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg agactgcagc
     4741 ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg actttgcttt
     4801 cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac
     4861 ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt ctcagcagct
     4921 gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca atgcggttta
     4981 aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt cttgctgtct
     5041 ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt cgttgagggt
     5101 cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat acatgggcat
     5161 aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt
     5221 gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag
     5281 caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt taagctggga
     5341 tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt tggctatgtt
     5401 cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag tgtatccggt
     5461 gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact tggagacgcc
     5521 cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg gcccacgggc
     5581 ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt ccaggatgag
     5641 atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg gtataatggt
     5701 tccatccggc ccaggggcgt agttaccctc acagatttgc atttcccacg ctttgagttc
     5761 agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg gggtagggga
     5821 gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc cggtgggccc
     5881 gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc tgccgtcatc
     5941 cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt ccctgaccaa
     6001 atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag caaagttttt
     6061 caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa gcagttccag
     6121 gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat ctcctcgttt
     6181 cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag acgggccagg
     6241 gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg
     6301 tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag
     6361 cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc
     6421 agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc gccgcacgag
     6481 gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga ttccggggag
     6541 taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca ggtgagctct
     6601 ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt cttacctctg
     6661 gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca
     6721 gacttgagag gcctgtcctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     6781 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     6841 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     6901 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     6961 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     7021 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     7081 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     7141 gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag
     7201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7741 cggcaaacaa accaccgctg gtagcggggg gttttttgtt tgcaagcagc agattacgcg
     7801 cagaaaaaaa ggatctcac
//
