Basic Vector Information
- Vector Name:
- pOst1-NubI
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8056 bp
- Type:
- Hybridization
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- TRP1
- Promoter:
- ADH1(medium)
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pOst1-NubI vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOst1-NubI vector Sequence
LOCUS 62056_18300 8056 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8056)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8056
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 904..1492
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 1780..1801
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1816..1846
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1854..1870
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1878..1894
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(1918..2105)
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
CDS complement(2283..2309)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(2319..2345)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(2367..3794)
/gene="OST1"
/label=OST1
/note="Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase subunit 1 from Saccharomyces cerevisiae
(strain ATCC 204508 / S288c). Accession#: P41543"
promoter complement(3798..4502)
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
primer_bind complement(4525..4541)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4682..5137
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(5243..5914)
/label=TRP1
/note="phosphoribosylanthranilate isomerase, required for
tryptophan biosynthesis"
promoter complement(5915..6196)
/label=TRP1 promoter
rep_origin 6455..7797
/label=2u ori
/note="yeast 2u plasmid origin of replication"
promoter 7824..7928
/label=AmpR promoter
CDS join(7929..8056,1..730)
/label=AmpR
/note="beta-lactamase"
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