LOCUS       V013450                 8056 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013450
VERSION     V013450
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      904..1492
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1780..1801
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1816..1846
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1854..1870
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1878..1894
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(1918..2105)
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2283..2309)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(2319..2345)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(2367..3794)
                     /gene="OST1"
                     /label="Dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 1"
                     /note="Dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 1 from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: P41543"
     promoter        complement(3798..4502)
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(4525..4541)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4682..5137
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5243..5914)
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     promoter        complement(5915..6196)
                     /label="TRP1 promoter"
     rep_origin      6455..7797
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        7824..7928
                     /label="AmpR promoter"
     CDS             join(7929..8056,1..730)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
       61 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1861 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgccg
     1921 gtagaggtgt ggtcaataag agcgacctca tgctatacct gagaaagcaa cctgacctac
     1981 aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat
     2041 ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa
     2101 ttcgcccgga attagcttgg ctgcaggcgg ccgctcatgg tcgacccacc agggattcct
     2161 tccttgtctt gaattttcga cttaacgttg tcgatggtat cggaagattc aacttccaat
     2221 gttatggttt taccggtcaa agtcttgacg aaaatctgca tggctgcagg tcgacggatc
     2281 tgagcgtaat ctggaacatc gtatgggtac tggatctgag cgtaatctgg aacatcgtat
     2341 gggtactgga tcccgagctc tgatccgtta gtaacgttca tgttcaaagt ttttaagaca
     2401 aaaactccca ttagtgcggt gaaaatatag caagcaatag acagaggctt cttgaaaaaa
     2461 gagcttttcg ggtagtcgta ctttatcaag acttggccat tggcaacttg actaatcaaa
     2521 tttctgtaac tgaaagttaa tttaacgtga cctttattta ggtcaaagta tgatttctgg
     2581 gtttctatag aaacatttgt aaaagggact ggagaatcaa tatcgaatat ttcggcccct
     2641 tccggaagaa ataccgataa ttcaacatta tcatatacag tgtccggtgg gccgtttaga
     2701 attgggatag aagcaacgaa tttctcgtct gagccagagg atacatgcaa gaaatcggac
     2761 aatttattag tccaaccgac agtaaaattg tagttccaac ctccaaagat tgggaatctt
     2821 ggtcttataa agaaatggtc acgttctgca tgcgacgtgg aaacaaggcc aaccaaatca
     2881 gtgaaataat gatcagtagc tccctcaggc aggagcatgt ctaagacagt aacaaagtga
     2941 gtttgtctca tattctgagt ttgaatctgt ttcattaatt ctaatcttga aaatccttta
     3001 gacagttttg cggctttgtt tgttaattca taatattcct caaattgtat tgtggaagcc
     3061 caatgagaaa gccaaatatc tcttctcaaa ttcactacct gattcaatgg ggcattgtgg
     3121 gaataaacaa ttgctaaggt ttcgtttgaa gaaaatctcg gaatatcttc ccaaggccca
     3181 aactcaaaag agtttccatt agcttttccc agtaaactct cgtcatttgg ggggtggtac
     3241 tcctcaaatg atgagctacc aatcagcgta aaagaggcct tcttggtatc gtaagcagaa
     3301 agcggcaatc tgttcgtttc ccacaatagg tgttgttctt ctgacattcc aacgtgctca
     3361 ggataaggaa tacctacggt attatagaag ctcttaatca ctaaagaaac ttcttcctga
     3421 ggggaaattg catttggaaa ttgaatgatc ccgtatctaa tttcactttc accatcgtca
     3481 ccaggtgctg tagtcgaatt ggcaagtaat tggctattta aaaaagttgc ctcgttggta
     3541 aaataggctg aaaaaaagga aactttactg aagatgccac tctcaaaggc tgtgaagtat
     3601 tcagtcgcag gttcgcttgc tatgtttttg atagttattt cgattgtttc tgaaatataa
     3661 gcgtttgaca cgtctattgt cctcttataa tcaacattct cccaagtcgc aggtggctcg
     3721 tattgggcag cagaagacac gttgaaaaaa cataggaaca atcccacaat ccaagagaac
     3781 caaatctgcc tcatatgagt tgattgtatg cttggtatag cttgaaatat tgtgcagaaa
     3841 aagaaacaag gaagaaaggg aacgagaaca atgacgagga aacaaaagat taataattgc
     3901 aggtctattt atacttgata gcaagacagc aaactttttt ttatttcaaa ttcaagtaac
     3961 tggaaggaag gccgtatacc gttgctcatt agagagtagt gtgcgtgaat gaaggaagga
     4021 aaaagtttcg tgtgcttcga gatacccctc atcagctctg gaacaacgac atctgttggt
     4081 gctgtctttg tcgttaattt tttcctttag tgtcttccat catttttttg tcattgcgga
     4141 tatggtgaga caacaacggg ggagagagaa aagaaaaaaa aagaaaagaa gttgcatgcg
     4201 cctattatta cttcaataga tggcaaatgg aaaaagggta gtgaaacttc gatatgatga
     4261 tggctatcaa gtctagggct acagtattag ttcgttatgt accaccatca atgaggcagt
     4321 gtaattggtg tagtcttgtt tagcccatta tgtcttgtct ggtatctgtt ctattgtata
     4381 tctcccctcc gccacctaca tgttagggag accaacgaag gtattatagg aatcccgatg
     4441 tatgggtttg gttgccagaa aagaggaagt ccatattgta cacccggaaa caacaaaagg
     4501 atatcgagct cgcgaaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4681 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4741 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4801 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4861 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4921 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4981 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5041 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5101 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     5161 cgcatctgtg cggtatttca caccgcatag atcggcaagt gcacaaacaa tacttaaata
     5221 aatactactc agtaataacc tatttcttag catttttgac gaaatttgct attttgttag
     5281 agtcttttac accatttgtc tccacacctc cgcttacatc aacaccaata acgccattta
     5341 atctaagcgc atcaccaaca ttttctggcg tcagtccacc agctaacata aaatgtaagc
     5401 tttcggggct ctcttgcctt ccaacccagt cagaaatcga gttccaatcc aaaagttcac
     5461 ctgtcccacc tgcttctgaa tcaaacaagg gaataaacga atgaggtttc tgtgaagctg
     5521 cactgagtag tatgttgcag tcttttggaa atacgagtct tttaataact ggcaaaccga
     5581 ggaactcttg gtattcttgc cacgactcat ctccatgcag ttggacgata tcaatgccgt
     5641 aatcattgac cagagccaaa acatcctcct taggttgatt acgaaacacg ccaaccaagt
     5701 atttcggagt gcctgaacta tttttatatg cttttacaag acttgaaatt ttccttgcaa
     5761 taaccgggtc aattgttctc tttctattgg gcacacatat aatacccagc aagtcagcat
     5821 cggaatctag agcacattct gcggcctctg tgctctgcaa gccgcaaact ttcaccaatg
     5881 gaccagaact acctgtgaaa ttaataacag acatactcca agctgccttt gtgtgcttaa
     5941 tcacgtatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc tctccttttc
     6001 ttttttcgac cgaattaatt cttaatcggc aaaaaaagaa aagctccgga tcaagattgt
     6061 acgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc atctggcgtc
     6121 ataactgcaa agtacacata tattacgatg ctgttctatt aaatgcttcc tatattatat
     6181 atatagtaat gtcgttgatc tatggtgcac tctcagtaca atctgctctg atgccgcata
     6241 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6301 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6361 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6421 ggttaatgtc atgataataa tggtttctta gtatgatcca atatcaaagg aaatgatagc
     6481 attgaaggat gagactaatc caattgagga gtggcagcat atagaacagc taaagggtag
     6541 tgctgaagga agcatacgat accccgcatg gaatgggata atatcacagg aggtactaga
     6601 ctacctttca tcctacataa atagacgcat ataagtacgc atttaagcat aaacacgcac
     6661 tatgccgttc ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg
     6721 aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa
     6781 cgctttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg
     6841 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
     6901 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
     6961 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
     7021 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
     7081 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
     7141 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
     7201 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
     7261 ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag
     7321 taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca
     7381 aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat
     7441 tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct
     7501 ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg
     7561 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
     7621 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
     7681 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     7741 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttcag
     7801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7921 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7981 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8041 tgggtgcacg agtggg
//
