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Cyto-roGFP vector (V013211)

Basic Vector Information

Vector Name:
Cyto-roGFP
Antibiotic Resistance:
Ampicillin
Length:
6935 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adenovirus
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

Cyto-roGFP vector Vector Map

Cyto-roGFP6935 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900oriAmpRAmpR promoterITRAd5 PsiCMV enhancerCMV promoterEGFPSV40 poly(A) signalIXIVa2

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Cyto-roGFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_611        6935 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(101..689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(863..1720)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1721..1825)
                     /label=AmpR promoter
     repeat_region   1939..2041
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     2129..2279
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        2308..2611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2612..2815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2870..3586
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(3610..3744)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4312..4731
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     CDS             complement(4797..6131)
                     /gene="IVa2"
                     /label=IVa2
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"

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