LOCUS       V013211                 6935 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013211
VERSION     V013211
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(101..689)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(863..1720)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1721..1825)
                     /label="AmpR promoter"
     repeat_region   1939..2041
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     2129..2279
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        2308..2611
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2612..2815
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2870..3586
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    complement(3610..3744)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             4312..4731
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(4797..6131)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
ORIGIN
        1 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc
       61 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      121 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      181 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      241 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      301 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      361 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      421 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      481 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      541 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      601 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      661 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      721 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      781 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      841 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      901 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      961 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1021 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1081 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1141 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     1201 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1261 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1321 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1381 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1441 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     1501 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1561 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1621 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1681 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1741 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1801 aaaataaaca aataggggtt ccgcgcactt ttcggggaaa tgtgccacct gacgtctaag
     1861 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     1921 ttcaattaat taagctagca tcatcaataa tataccttat tttggattga agccaatatg
     1981 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
     2041 gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg
     2101 gcaaaagtga cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt
     2161 tttaggcgga tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg
     2221 gaaaactgaa taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata
     2281 tttgtctagg gagatcagcc tgcaggtcgt tacataactt acggtaaatg gcccgcctgg
     2341 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2401 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2461 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2521 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2581 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2641 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2701 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2761 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2821 agtgaaccgt cagatggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
     2881 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2941 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3001 cctgaagttc atcagcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3061 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc ggcacgactt
     3121 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3181 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3241 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3301 caactacaac tgccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3361 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3421 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3481 ctgctccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3541 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcca
     3601 cagcggggag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3661 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3721 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3781 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     3841 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3901 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3961 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagtcc
     4021 ccgcggtggc agatctggaa ggtgctgagg tacgatgaga cccgcaccag gtgcagaccc
     4081 tgcgagtgtg gcggtaaaca tattaggaac cagcctgtga tgctggatgt gaccgaggag
     4141 ctgaggcccg atcacttggt gctggcctgc acccgcgctg agtttggctc tagcgatgaa
     4201 gatacagatt gaggtactga aatgtgtggg cgtggcttaa gggtgggaaa gaatatataa
     4261 ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc
     4321 aactcgtttg atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc
     4381 ggggtgcgtc agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac
     4441 tctactacct tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc
     4501 gccgcttcag ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc
     4561 ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt
     4621 ttggcacaat tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat
     4681 ctgcgccagc aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata
     4741 aataaaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta
     4801 ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt
     4861 attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg
     4921 tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg
     4981 atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg
     5041 attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc
     5101 atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc
     5161 atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg
     5221 ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga
     5281 cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc
     5341 tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca
     5401 taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc
     5461 ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg
     5521 gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc
     5581 tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc
     5641 acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc
     5701 aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc
     5761 agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt
     5821 ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc
     5881 cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt
     5941 tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt
     6001 ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc
     6061 cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc
     6121 ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct
     6181 ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt
     6241 gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat
     6301 ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt
     6361 cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca
     6421 tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga
     6481 gaggcctgtc ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     6541 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     6601 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     6661 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     6721 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     6781 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     6841 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     6901 aggcaggtag atgacgacca tcagggacag cttca
//
