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pPICZαA-SIRT4 vector (V013206)

Basic Vector Information

Vector Name:
pPICZαA-SIRT4
Antibiotic Resistance:
Bleomycin
Length:
4532 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
Zeo
Promoter:
AOX1
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pPICZαA-SIRT4 vector Vector Map

pPICZαA-SIRT44532 bp600120018002400300036004200AOX1 promoteralpha-factor secretion signalNAD-dependent protein lipoamidase sirtuin-4, mitochondrialMyc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPICZαA-SIRT4 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V013206                 4532 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013206
VERSION     V013206
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label="alpha-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             1265..2206
                     /gene="SIRT4"
                     /label="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial"
                     /note="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial from Homo sapiens. Accession#: Q9Y6E7"
     CDS             2214..2243
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2259..2276
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2356..2602
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        2617..3028
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        3036..3083
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3102..3473
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      3542..3789
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3864..4452)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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