LOCUS       V013206                 4532 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013206
VERSION     V013206
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label="alpha-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             1265..2206
                     /gene="SIRT4"
                     /label="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial"
                     /note="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial from Homo sapiens. Accession#: Q9Y6E7"
     CDS             2214..2243
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2259..2276
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2356..2602
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        2617..3028
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        3036..3083
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3102..3473
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      3542..3789
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3864..4452)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgaa ttcacgtggc ccagccggcc gtctcggatc ggtacctcga gccgcggcgg
     1261 ccgcatgaag atgagctttg cgttgacttt caggtcagca aaaggccgtt ggatcgcaaa
     1321 ccccagccag ccgtgctcga aagcctccat tgggttattt gtgccagcaa gtcctcctct
     1381 ggaccctgag aaggtcaaag agttacagcg cttcatcacc ctttccaaga gactccttgt
     1441 gatgactggg gcaggaatct ccaccgaatc ggggatacca gactacaggt cagaaaaagt
     1501 ggggctttat gcccgcactg accgcaggcc catccagcat ggtgattttg tccggagtgc
     1561 cccaatccgc cagcggtact gggcgagaaa cttcgtaggc tggcctcaat tctcctccca
     1621 ccagcctaac cctgcacact gggctttgag cacctgggag aaactcggaa agctgtactg
     1681 gttggtgacc caaaatgtgg atgctttgca caccaaggcg gggagtcggc gcctgacaga
     1741 gctccacgga tgcatggaca gggtcctgtg cttggattgt ggggaacaga ctccccgggg
     1801 ggtgctgcaa gagcgtttcc aagtcctgaa ccccacctgg agtgctgagg cccatggcct
     1861 ggctcctgat ggtgacgtct ttctctcaga ggagcaagtc cggagctttc aggtcccaac
     1921 ctgcgttcaa tgtggaggcc atctgaaacc agatgtcgtt ttcttcgggg acacagtgaa
     1981 ccctgacaag gttgattttg tgcacaagcg tgtaaaagaa gccgactccc tcttggtggt
     2041 gggatcatcc ttgcaggtat actctggtta caggtttatc ctcactgcct gggagaagaa
     2101 gctcccgatt gcaatactga acattgggcc cacacggtcg gatgacttgg cgtgtctgaa
     2161 actgaattct cgttgtggag agttgctgcc tttgatagac ccatgctgat ctagaacaaa
     2221 aactcatctc agaagaggat ctgaatagcg ccgtcgacca tcatcatcat catcattgag
     2281 tttgtagcct tagacatgac tgttcctcag ttcaagttgg gcacttacga gaagaccggt
     2341 cttgctagat tctaatcaag aggatgtcag aatgccattt gcctgagaga tgcaggcttc
     2401 atttttgata cttttttatt tgtaacctat atagtatagg attttttttg tcattttgtt
     2461 tcttctcgta cgagcttgct cctgatcagc ctatctcgca gctgatgaat atcttgtggt
     2521 aggggtttgg gaaaatcatt cgagtttgat gtttttcttg gtatttccca ctcctcttca
     2581 gagtacagaa gattaagtga gaccttcgtt tgtgcggatc ccccacacac catagcttca
     2641 aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac
     2701 cacttcaaaa cacccaagca cagcatacta aattttccct ctttcttcct ctagggtgtc
     2761 gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt ttctttttct
     2821 tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaat tttttttttt
     2881 agtttttttc tctttcagtg acctccattg atatttaagt taataaacgg tcttcaattt
     2941 ctcaagtttc agtttcattt ttcttgttct attacaactt tttttacttc ttgttcatta
     3001 gaaagaaagc atagcaatct aatctaaggg gcggtgttga caattaatca tcggcatagt
     3061 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     3121 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     3181 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     3241 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     3301 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     3361 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     3421 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     3481 tccgacggcg gcccacgggt cccaggcctc ggagatccgt cccccttttc ctttgtcgat
     3541 atcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     3601 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     3661 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     3721 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     3781 ttaatttgca agctggagac caacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4021 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     4081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4501 aactcacgtt aagggatttt ggtcatgaga tc
//
