Basic Vector Information
- Vector Name:
- pPICZA-SIRT4
- Antibiotic Resistance:
- Bleomycin
- Length:
- 4269 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- Zeo
- Promoter:
- AOX1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pPICZA-SIRT4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPICZA-SIRT4 vector Sequence
LOCUS V013204 4269 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V013204 VERSION V013204 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 4269) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4269 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..940 /label="AOX1 promoter" /note="inducible promoter, regulated by methanol" CDS 987..1928 /gene="SIRT4" /label="NAD-dependent protein lipoamidase sirtuin-4, mitochondrial" /note="NAD-dependent protein lipoamidase sirtuin-4, mitochondrial from Homo sapiens. Accession#: Q9Y6E7" CDS 1952..1981 /label="Myc" /note="Myc (human c-Myc proto-oncogene) epitope tag" CDS 1997..2014 /label="6xHis" /note="6xHis affinity tag" terminator 2093..2339 /label="AOX1 terminator" /note="transcription terminator for AOX1" promoter 2354..2765 /label="TEF1 promoter" /note="promoter for EF-1-alpha" promoter 2773..2820 /label="EM7 promoter" /note="synthetic bacterial promoter" CDS 2839..3210 /label="BleoR" /note="antibiotic-binding protein" terminator 3279..3526 /label="CYC1 terminator" /note="transcription terminator for CYC1" rep_origin complement(3601..4189) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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