LOCUS       V013204                 4269 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013204
VERSION     V013204
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             987..1928
                     /gene="SIRT4"
                     /label="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial"
                     /note="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial from Homo sapiens. Accession#: Q9Y6E7"
     CDS             1952..1981
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1997..2014
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2093..2339
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        2354..2765
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        2773..2820
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2839..3210
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      3279..3526
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3601..4189)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggcccagc
      961 cggccgtctc ggatcggtac ctcgagatga agatgagctt tgcgttgact ttcaggtcag
     1021 caaaaggccg ttggatcgca aaccccagcc agccgtgctc gaaagcctcc attgggttat
     1081 ttgtgccagc aagtcctcct ctggaccctg agaaggtcaa agagttacag cgcttcatca
     1141 ccctttccaa gagactcctt gtgatgactg gggcaggaat ctccaccgaa tcggggatac
     1201 cagactacag gtcagaaaaa gtggggcttt atgcccgcac tgaccgcagg cccatccagc
     1261 atggtgattt tgtccggagt gccccaatcc gccagcggta ctgggcgaga aacttcgtag
     1321 gctggcctca attctcctcc caccagccta accctgcaca ctgggctttg agcacctggg
     1381 agaaactcgg aaagctgtac tggttggtga cccaaaatgt ggatgctttg cacaccaagg
     1441 cggggagtcg gcgcctgaca gagctccacg gatgcatgga cagggtcctg tgcttggatt
     1501 gtggggaaca gactccccgg ggggtgctgc aagagcgttt ccaagtcctg aaccccacct
     1561 ggagtgctga ggcccatggc ctggctcctg atggtgacgt ctttctctca gaggagcaag
     1621 tccggagctt tcaggtccca acctgcgttc aatgtggagg ccatctgaaa ccagatgtcg
     1681 ttttcttcgg ggacacagtg aaccctgaca aggttgattt tgtgcacaag cgtgtaaaag
     1741 aagccgactc cctcttggtg gtgggatcat ccttgcaggt atactctggt tacaggttta
     1801 tcctcactgc ctgggagaag aagctcccga ttgcaatact gaacattggg cccacacggt
     1861 cggatgactt ggcgtgtctg aaactgaatt ctcgttgtgg agagttgctg cctttgatag
     1921 acccatgctg agcggccgcc agcttgggcc cgaacaaaaa ctcatctcag aagaggatct
     1981 gaatagcgcc gtcgaccatc atcatcatca tcattgagtt ttagccttag acatgactgt
     2041 tcctcagttc aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg
     2101 atgtcagaat gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt
     2161 aacctatata gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct
     2221 gatcagccta tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga
     2281 gtttgatgtt tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac
     2341 cttcgtttgt gcggatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac
     2401 tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag
     2461 catactaaat tttccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt
     2521 tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat
     2581 ttttatcacg tttctttttc ttgaaatttt tttttttagt ttttttctct ttcagtgacc
     2641 tccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc
     2701 ttgttctatt acaacttttt ttacttcttg ttcattagaa agaaagcata gcaatctaat
     2761 ctaaggggcg gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     2821 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     2881 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     2941 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     3001 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     3061 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     3121 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     3181 tgcgtgcact tcgtggccga ggagcaggac tgacacgtcc gacggcggcc cacgggtccc
     3241 aggcctcgga gatccgtccc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg
     3301 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     3361 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     3421 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     3481 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc tggagaccaa
     3541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4261 catgagatc
//
