Basic Vector Information
- Vector Name:
- (-30)GFP-OspF
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6706 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
(-30)GFP-OspF vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
(-30)GFP-OspF vector Sequence
LOCUS 62056_6 6706 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6706)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6706
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 3..27
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 42..64
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 887..1600
/gene="ospF"
/label=ospF
/note="Phosphothreonine lyase OspF from Shigella flexneri.
Accession#: Q8VSP9"
CDS 1616..1633
/label=6xHis
/note="6xHis affinity tag"
CDS 1652..1696
/label=S-Tag
/note="affinity and epitope tag derived from pancreatic
ribonuclease A"
terminator 1748..1795
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1832..2287
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(2405..3262)
/label=AmpR
/note="beta-lactamase"
promoter complement(3263..3355)
/label=AmpR promoter
rep_origin 3436..4024
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(4210..4352)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4457..4645)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(5183..5204)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(5220..6299)
/label=lacI
/note="lac repressor"
promoter complement(6300..6377)
/label=lacI promoter
promoter 6690..6706
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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