LOCUS       V013097                 6706 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013097
VERSION     V013097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             887..1600
                     /gene="ospF"
                     /label="Phosphothreonine lyase OspF"
                     /note="Phosphothreonine lyase OspF from Shigella flexneri.
                     Accession#: Q8VSP9"
     CDS             1616..1633
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1652..1696
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      1748..1795
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1832..2287
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2405..3262)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3263..3355)
                     /label="AmpR promoter"
     rep_origin      3436..4024
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4210..4352)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4457..4645)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(5183..5204)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5220..6299)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6300..6377)
                     /label="lacI promoter"
     promoter        6690..6706
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggtgcta gcaaaggtga agagctgttt gacggtgtag taccgatctt
      121 agtggaatta gacggcgacg tgaacggtca cgaatttagc gtgcgcggcg agggcgaagg
      181 tgacgctacc gagggtgaat tgaccctgaa gtttatttgc acaacaggcg aattacccgt
      241 tccgtggccc accttagtga ccaccctgac ctatggcgtt cagtgcttca gtgattaccc
      301 agatcatatg gatcaacacg attttttcaa atcagccatg cctgaaggat atgttcaaga
      361 gcgtacaatc agcttcaagg acgatggcac ctataaaacg cgtgcggaag tgaaatttga
      421 aggcgacaca ttagtaaacc gtatcgaact gaaaggtatc gacttcaaag aagacggcaa
      481 cattttaggc cataagctgg aatataactt taattctcat gacgtgtata ttacggccga
      541 taaacaggaa aacggtatca aggcagaatt tgaaattcgc cataacgtgg aggacggcag
      601 cgttcaatta gcggatcatt atcaacaaaa cacgccgatt ggtgatgggc ctgtactgtt
      661 acctgacgat cactacctga gcacggagtc agccctgagc aaagatccga acgaagaccg
      721 cgatcacatg gttctgttag aattcgtgac cgctgcaggc attgatcatg gaatggacga
      781 gctgtacaag accggtggta gcggtggttc tggtggttct ggtggtagcg gcggtagcgg
      841 tggtagcggt ggtagcggtg gcagcggcgg taccgcgagc atggctccca taaaaaagcc
      901 ctgtctaaaa ctaaatttag attctttgaa tgttgtgagg tctgaaatac cgcagatgct
      961 ttctgcgaat gaaagattga aaaataactt caatatattg tacaaccaaa tacggcaata
     1021 cccagcgtac tatttcaaag tagcatcgaa cgtgccaact tactctgata tatgtcaatc
     1081 tttttctgtc atgtaccaag gattccagat tgtgaaccat agtggagatg tgtttattca
     1141 tgcatgtcgt gaaaatcctc agagtaaggg ggattttgtt ggggacaagt ttcatattag
     1201 tatagctagg gagcaagttc ctctggcatt ccaaattttg tctggtttat tattttcaga
     1261 ggacagtcct atagataaat ggaagataac tgatatgaat cgagtttctc agcaatctcg
     1321 tgtggggata ggagctcagt ttacgctata tgtaaaatca gatcaggaat gctcgcaata
     1381 tagtgcttta ttacttcata aaatacgaca atttataatg tgtctcgagt ctaatctatt
     1441 aagaagcaaa atcgctcccg gggagtatcc ggcgtcagat gttagacctg aagactggaa
     1501 atatgtcagc tatcgtaatg aactacgaag cgatcgagat ggaagtgaaa ggcaagagca
     1561 aatgttacga gaggaaccat tttatcgttt gatgatagag tgagacggcg gatcccatca
     1621 ccaccaccat cactaatgac tcgagtctgg taaagaaacc gctgctgcga aatttgaacg
     1681 ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga
     1741 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1801 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     1861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     2281 acaatttctg gcggcacgat ggcatgagat tatcaaaaag gatcttcacc tagatccttt
     2341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3241 cacggaaatg ttgaatactc atactcttcc tttttcaatc atgattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggt
     3361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4201 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4261 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     4321 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     4381 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4441 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     4501 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     4561 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     4621 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     4681 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     4741 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     4801 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     4861 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     4921 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     4981 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     5041 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     5101 tgctagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     5161 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     5221 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5281 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     5341 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     5401 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     5461 gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg
     5521 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     5581 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     5641 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     5701 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     5761 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     5821 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     5881 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     5941 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     6001 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     6061 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     6121 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     6181 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     6241 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     6301 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     6361 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     6421 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     6481 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     6541 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     6601 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     6661 gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actata
//
