Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012802 | pGro7 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
Plasmids of chaperone. The function of the plasmid pGro7 is to express the GroEL and GroES proteins, which are chaperonins involved in protein folding in the cytoplasm of Escherichia coli. pGro7 is a high-copy-number plasmid, meaning that it exists in multiple copies per cell, and it is often used for overexpression of proteins in E. coli. The GroEL and GroES proteins form a complex that helps to fold newly synthesized proteins into their correct conformation, preventing protein aggregation and enhancing protein stability. pGro7 is a valuable tool in molecular biology for protein production and research.
- Vector Name:
- pGro7
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 5467 bp
- Type:
- E.coli expression plasmid
- Replication origin:
- p15A ori
- Source/Author:
- Takara
- Copy Number:
- Medium copy number
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pGro7 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Pei C, Peng X, Wu Y, Jiao R, Li T, Jiao S, Zhou L, Li J, Du Y, Qian EW. Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli. Enzyme Microb Technol. 2024 Mar 12;177:110426. doi: 10.1016/j.enzmictec.2024.110426. Epub ahead of print. PMID: 38503081.
pGro7 vector Sequence
LOCUS pGro7. 5467 bp DNA circular SYN 10-JUN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pGro7. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5467) TITLE Direct Submission FEATURES Location/Qualifiers source 1..5467 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 1862..1915 /label=S loop /note="GroES chaperone mobile loop that interacts with GroEL" CDS 2154..3797 /gene="groEL" /label=groEL /note="Chaperonin GroEL from Escherichia coli O8 (strain IAI1). Accession#: B7M8Q4" rep_origin 4076..4620 /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." promoter 5146..5248 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS join(5249..5467,1..438) /label=CmR /note="chloramphenicol acetyltransferase"