LOCUS       Exported                5467 bp DNA     circular SYN 28-NOV-2025
DEFINITION  Exported.
ACCESSION   V012802
VERSION     .
KEYWORDS    pGro7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1860..1913
                     /label=S loop
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
     CDS             2152..3795
                     /gene="groEL"
                     /label=Chaperonin GroEL
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     rep_origin      4075..4620
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        5146..5248
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(5249..5467,1..438)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa tagttacggc
      541 ttatgacatc tttgtggaca catcattcac tttttattca catccggccc tgaactcgct
      601 aggacttgcc ccggtgcatt ttttaaatac ccgcgaaaaa tagagctgat cgtcaaatcc
      661 aacattgcgc ccaacggtcg ctatcggcat tcgcgtagtg ctaagcagaa gtttcgcctg
      721 gctgatacgc tgatcttcgc gccagctcaa tacgctaatg cctaactgct ggcggaacag
      781 atgtgataac cgggagggcg acaggcagac atgctgggcg acgctggcga tatcaaaatg
      841 gctgtccgcc agatggtcgc tgatatactg gcaggcatcg cgcacacggc tatccatcgg
      901 cgggtgcaac gactcattaa ttaccgccat acgtctgagc aacaactgct ccagcagatt
      961 gatcgccagt agctcagaat agcgaccttc cccttgcccg gcgctgatga tctgcccgaa
     1021 cagttcgctg aaatgcggct ggcgcgcctc gtccgggcgg aaaaatcctg tctgggcaaa
     1081 gattgtcggc caggtcagcc actcctgcca gtaggcgcga ggccggaaat aaacccactg
     1141 gtgataccac tcgctggcgt ccggatgccg tccatagtga tgaatctcgc ccggcggaaa
     1201 caataatata tcgccaggcc gacagacaaa ctgctcgcca ttattattaa tgacgccctc
     1261 tccgcggatg gtcaggttaa gaatatatcc cttcatgccc aacggacgat cgataaaaaa
     1321 atccagatat ccattcgctt caattggcgt cagcccggcg accagatggg cattaaatga
     1381 atatcccggc aatagcggat cattttgcgt ttcagccatg atttctctac cccccgatgt
     1441 tcagagaaga aacaaattgt ccatatcgac caggacgaca gagcttccgt ctccgcaaga
     1501 ctttgcgctt gatgaaagca cgtatcaacc ccgcttgtga aaagcgcttt gtaacaaaag
     1561 cgtacagttc aggcgataaa attaagtaac agaagtgtct ataactatgg ctggaatgtc
     1621 cacattgaat atttgcacag cgtcacactt tgcaaagcat tagcattttt gtccataaga
     1681 ttagcggatc ctgcctgacg gtttttgccg cgactctcta taatttctcc atacctgttt
     1741 ttctggatgg agtaagacca tggctcgagg cgtcacccat aacagatacg gactttctca
     1801 aaggagagtt atcaatgaat attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag
     1861 aagttgaaac taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca
     1921 cccgcggcga agtgctggct gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc
     1981 cgctggatgt gaaagttggc gacatcgtta ttttcaacga tggctacggt gtgaaatctg
     2041 agaagatcga caatgaagaa gtgttgatca tgtccgaaag cgacattctg gcaattgttg
     2101 aagcgtaatc cgcgcacgac actgaacata cgaatttaag gaataaagat aatggcagct
     2161 aaagacgtaa aattcggtaa cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg
     2221 gcagatgcag tgaaagttac cctcggtcca aaaggccgta acgtagttct ggataaatct
     2281 ttcggtgcac cgaccatcac caaagatggt gtttccgttg ctcgtgaaat cgaactggaa
     2341 gacaagttcg aaaatatggg tgcgcagatg gtgaaagaag ttgcctctaa agcaaacgac
     2401 gctgcaggcg acggtaccac cactgcaacc gtactggctc aggctatcat cactgaaggt
     2461 ctgaaagctg ttgctgcggg catgaacccg atggacctga aacgtggtat cgacaaagcg
     2521 gttaccgctg cagttgaaga actgaaagcg ctgtccgtac catgctctga ctctaaagcg
     2581 attgctcagg ttggtaccat ctccgctaac tccgacgaaa ccgtaggtaa actgatcgct
     2641 gaagcgatgg acaaagtcgg taaagaaggc gttatcaccg ttgaagacgg taccggtctg
     2701 caggacgaac tggacgtggt tgaaggtatg cagttcgacc gtggctacct gtctccttac
     2761 ttcatcaaca agccggaaac tggcgcagta gaactggaaa gcccgttcat cctgctggct
     2821 gacaagaaaa tctccaacat ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca
     2881 ggcaaaccgc tgctgatcat cgctgaagat gtagaaggcg aagcgctggc aactctggtt
     2941 gttaacacca tgcgtggcat cgtgaaagtc gctgcggtta aagcaccggg cttcggcgat
     3001 cgtcgtaaag ctatgctgca ggatatcgca accctgactg gcggtaccgt gatctctgaa
     3061 gagatcggta tggagctgga aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt
     3121 gtgatcaaca aagacaccac cactatcatc gatggcgtgg gtgaagaagc tgcaatccag
     3181 ggccgtgttg ctcagatccg tcagcagatt gaagaagcaa cttctgacta cgaccgtgaa
     3241 aaactgcagg aacgcgtagc gaaactggca ggcggcgttg cagttatcaa agtgggtgct
     3301 gctaccgaag ttgaaatgaa agagaaaaaa gcacgcgttg aagatgccct gcacgcgacc
     3361 cgtgctgcgg tagaagaagg cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg
     3421 tctaaactgg ctgacctgcg tggtcagaac gaagaccaga acgtgggtat caaagttgca
     3481 ctgcgtgcaa tggaagctcc gctgcgtcag atcgtattga actgcggcga agaaccgtct
     3541 gttgttgcta acaccgttaa aggcggcgac ggcaactacg gttacaacgc agcaaccgaa
     3601 gaatacggca acatgatcga catgggtatc ctggatccaa ccaaagtaac tcgttctgct
     3661 ctgcagtacg cagcttctgt ggctggcctg atgatcacca ccgaatgcat ggttaccgac
     3721 ctgccgaaaa acgatgcagc tgacttaggc gctgctggcg gtatgggcgg catgggtggc
     3781 atgggcggca tgatgtaatt gccctgcacc tcgcagaaat aaacaaaccc ccgggcagaa
     3841 atgtctgggg gtttttcttt tggtcatctt tctagtataa gattcagaca cggacgacgc
     3901 gagtggcgcc tcgaggcgcc gatatcagat ctggtaccaa gcttatcgat gataagctgt
     3961 caaacatgag aattacaact tatatcgtat ggggctgact tcaggtgcta catttgaaga
     4021 gataaattgc actgaaatct agaaatattt tatctgatta ataagatgat cttcttgaga
     4081 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg
     4141 tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     4201 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa
     4261 ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt
     4321 tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     4381 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     4441 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     4501 cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4561 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     4621 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     4681 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     4741 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     4801 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     4861 gtaagccagt atacactccg ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc
     4921 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
     4981 caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccgacg cactttgcgc
     5041 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
     5101 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
     5161 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     5221 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     5281 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     5341 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     5401 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     5461 ggaattc
//
