RNAi-Ready pSIREN-RetroQ-ZsGreen1 vector (V012740)

MSDS

Basic Vector Information

Vector Name:: RNAi-Ready pSIREN-RetroQ-ZsGreen1

Antibiotic Resistance:: Ampicillin

Length:: 6562 bp

Type:: RNAi

Replication origin:: ori

Source/Author:: Clontech

Selection Marker:: ZsGreen1

Copy Number:: Low copy number

Promoter:: U6

Expression Method:: Transient

RNAi-Ready pSIREN-RetroQ-ZsGreen1 vector Vector Map

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RNAi-Ready pSIREN-RetroQ-ZsGreen1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Untitled.        6562 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6562)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             618..1310
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     LTR             1433..1858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        2124..2453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2746..3334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3508..4365)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4366..4470)
                     /label=AmpR promoter
     enhancer        4656..5035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5036..5239
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             5240..5415
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5478..5677
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5878..6294
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        6318..6558
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"

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