Basic Vector Information
RNAi-Ready pSIREN-RetroQ-ZsGreen1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
RNAi-Ready pSIREN-RetroQ-ZsGreen1 vector Sequence
LOCUS Untitled. 6562 bp DNA circular SYN 11-SEP-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS Untitled. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6562) TITLE Direct Submission REFERENCE 2 (bases 1 to 6562) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6562 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 66..369 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 370..573 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 618..1310 /label=ZsGreen1 /note="Zoanthus green fluorescent protein" LTR 1433..1858 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" promoter 2124..2453 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin complement(2746..3334) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3508..4365) /label=AmpR /note="beta-lactamase" promoter complement(4366..4470) /label=AmpR promoter enhancer 4656..5035 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5036..5239 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" LTR 5240..5415 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 5478..5677 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 5878..6294 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" promoter 6318..6558 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA"
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