LOCUS       Untitled.        6562 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             618..1310
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     LTR             1433..1858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        2124..2453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2746..3334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3508..4365)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4366..4470)
                     /label=AmpR promoter
     enhancer        4656..5035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5036..5239
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             5240..5415
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5478..5677
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5878..6294
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        6318..6558
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
ORIGIN
        1 aattctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      601 cgctaccggt cgccaccatg gcccagtcca agcacggcct gaccaaggag atgaccatga
      661 agtaccgcat ggagggctgc gtggacggcc acaagttcgt gatcaccggc gagggcatcg
      721 gctacccctt caagggcaag caggccatca acctgtgcgt ggtggagggc ggccccttgc
      781 ccttcgccga ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg ttcaccgagt
      841 acccccagga catcgtcgac tacttcaaga actcctgccc cgccggctac acctgggacc
      901 gctccttcct gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc accgtgagcg
      961 tggaggagaa ctgcatgtac cacgagtcca agttctacgg cgtgaacttc cccgccgacg
     1021 gccccgtgat gaagaagatg accgacaact gggagccctc ctgcgagaag atcatccccg
     1081 tgcccaagca gggcatcttg aagggcgacg tgagcatgta cctgctgctg aaggacggtg
     1141 gccgcttgcg ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc cgcaagatgc
     1201 ccgactggca cttcatccag cacaagctga cccgcgagga ccgcagcgac gccaagaacc
     1261 agaagtggca cctgaccgag cacgccatcg cctccggctc cgccttgccc taactcgaga
     1321 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     1381 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     1441 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     1501 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
     1561 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     1621 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     1681 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     1741 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     1801 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     1861 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     1921 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1981 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2041 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     2101 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2281 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     2401 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     2461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     2521 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     2581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     4141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4561 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat
     4621 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
     4681 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     4741 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     4801 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     4861 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     4921 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     4981 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     5041 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     5101 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     5161 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     5221 ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct
     5281 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
     5341 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga
     5401 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg
     5461 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
     5521 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg
     5581 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc
     5641 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
     5701 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
     5761 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
     5821 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
     5881 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     5941 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     6001 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     6061 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     6121 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     6181 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     6241 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt
     6301 gaagatctgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     6361 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     6421 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     6481 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     6541 atatcttgtg gaaaggacga gg
//
