Basic Vector Information
- Vector Name:
- pMET A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7778 bp
- Type:
- Yeast Expression Vectors
- Replication origin:
- ori
- Selection Marker:
- ADE2
- Copy Number:
- High copy number
- Promoter:
- ADE2
- Cloning Method:
- Enzyme digestion and ligation
- 5' Primer:
- AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´
- 3' Primer:
- AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
- Fusion Tag:
- C-V5, C-His
pMET A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMET A vector Sequence
LOCUS 40924_30695 7778 bp DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7778) TITLE Direct Submission REFERENCE 2 (bases 1 to 7778) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..7778 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 1268..1309 /codon_start=1 /label=V5 tag /note="epitope tag from simian virus 5" /translation="GKPIPNPLLGLDST" CDS 1319..1336 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" promoter 2444..2819 /label=ADE2 promoter rep_origin complement(5750..6338) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6512..7369) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7370..7474) /label=AmpR promoter
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