LOCUS       40924_30695        7778 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1268..1309
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1319..1336
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        2444..2819
                     /label=ADE2 promoter
     rep_origin      complement(5750..6338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6512..7369)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7370..7474)
                     /label=AmpR promoter
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcgagagga
     1201 attcacgtgg cccagccggc cgtggatcca cgcgtcgtcg acccgcggcg gccgccagct
     1261 tcctaggggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     1321 tcatcaccat caccattgaa ctagtataca attctagggc tgcctgtttg gatattttta
     1381 taatttttga gagtttgcca actaatgttt ttctcttcta tgatatttat catgtagttg
     1441 gatatctttt tttctttgaa aagtttttat ttagttcttt ctctcttttt tataatgttt
     1501 tcagtttctt tcctttatat tgaaaatacg atgttctttg tctttctttt ttaaatcctc
     1561 aattataatg tgtaccgttt gatcaacttg taaaattgtt tgtagtcctg taatcttaat
     1621 tgacgattgg ttgatagttt accccagtat attgtgcggc tgagatatta cccgattcgg
     1681 aagattgatt ttttcaacag acataaaccc ataggagatt tagtacagcg acatatgcta
     1741 attgcctgat aatgctcact aacattttct ccttttaatc tggtatatgc atacaggtat
     1801 gttgatatgg ccttgttgat caaagctacg aaagtgtaga ctatttccca aacagccccc
     1861 aaccaccacc tcacacgcgc acactgcgaa gtgaaagcgc tcccttattt tcttctccgc
     1921 tggttcaaaa caaaaaagaa tctaatctgt agacacacgc cccgtaaaac ccaatcttca
     1981 tcatcgtaat tttcagtgac ggacgatcga tcaaccatat agtttgttag tgactgagct
     2041 tcagtagttg acctcttgaa gaaataacgt cttgaacaac atactttata cctaccaaca
     2101 ttcgccatca acaacagtgt gacactgaca taactttaca tccattcatc gacaaaaaaa
     2161 tgtctctagt tcggattcat gcttatgggt tagctatttc gcccaatgtg tccatctgac
     2221 attactattt tgcattttaa tttaattaga acttgactag cgcactacca gcatatcatc
     2281 tcatttccgt aaataccaaa tgtattatat attgaaagct tttgaccagg ttattataaa
     2341 agaaacttca tgctcgaaaa agatcatttc gaaaagttgc ctagtttcat gaaattttaa
     2401 agcagtttat ataaatttta ccttttgatg cggaattgac tttttcttga ataatacata
     2461 acttttctta aaagaatcaa agacagataa aatttaagag atattaaata ttagtgagaa
     2521 gccgagaatt ttgtaacacc aacataacac tgacatcttt aacaactttt aattatgata
     2581 catttcttac gtcatgattg attattacag ctatgctgac aaatgactct tgttgcatgg
     2641 ctacgaaccg ggtaatacta agtgattgac tcttgctgac cttttattaa gaactaaatg
     2701 gacaatatta tggagcattt catgtataaa ttggtgcgta aaatcgttgg atctctcttc
     2761 taagtacatc ctactataac aatcaagaaa aacaagaaaa tcggacaaaa caatcaagta
     2821 tggattctag aacagttggt atattaggag ggggacaatt gggacgtatg attgttgagg
     2881 cagcaaacag gctcaacatt aagacggtaa tactagatgc tgaaaattct cctgccaaac
     2941 aaataagcaa ctccaatgac cacgttaatg gctccttttc caatcctctt gatatcgaaa
     3001 aactagctga aaaatgtgat gtgctaacga ttgagattga gcatgttgat gttcctacac
     3061 taaagaatct tcaagtaaaa catcccaaat taaaaattta cccttctcca gaaacaatca
     3121 gattgataca agacaaatat attcaaaaag agcatttaat caaaaatggt atagcagtta
     3181 cccaaagtgt tcctgtggaa caagccagtg agacgtccct attgaatgtt ggaagagatt
     3241 tgggttttcc attcgtcttg aagtcgagga ctttggcata cgatggaaga ggtaacttcg
     3301 ttgtaaagaa taaggaaatg attccggaag ctttggaagt actgaaggat cgtcctttgt
     3361 cgccgaaaaa tgggcaccat ttactaaaga attagcagtc atgattgtga gatctgttaa
     3421 cggtttagtg ttttcttacc caattgtaga gactatccac aaggacaata tttgtgactt
     3481 atgttatgcg cctgctagag ttccggactc cgttcaactt aaggcgaagt tgttggcaga
     3541 aaatgcaatc aaatcttttc ccggttgtgg tatatttggt gtggaaatgt tctatttaga
     3601 aacaggggaa ttgcttatta acgaaattgc cccaaggcct cacaactctg gacattatac
     3661 cattgatgct tgcgtcactt ctcaatttga agctcatttg agatcaatat tggatttgcc
     3721 aatgccaaag aatttcacat ctttctccac cattacaacg aacgccatta tgctaaatgt
     3781 tcttggagac aaacatacaa aagataaaga gctagaaact tgcgaaagag cattggcgac
     3841 tccaggttcc tcagtgtact tatatggaaa agagtctaga cctaacagaa aagtaggtca
     3901 cataaatatt attgcctcca gtatggcgga atgtgaacaa aggctgaact acattacagg
     3961 tagaactgat attccaatca aaatctctgt cgctcaaaag ttggacttgg aagcaatggt
     4021 caaaccattg gttggaatca tcatgggatc agactctgac ttgccggtaa tgtctgccgc
     4081 atgtgcggtt ttaaaagatt ttggcgttcc atttgaagtg acaatagtct ctgctcatag
     4141 aactccacat aggatgtcag catatgctat ttccgcaagc aagcgtggaa ttaaaacaat
     4201 tatcgctgga gctggtgggg ctgctcactt gccaggtatg gtggctgcaa tgacaccact
     4261 tcctgtcatc ggtgtgcccg taaaaggttc ttgtctagat ggagtagatt ctttacattc
     4321 aattgtgcaa atgcctagag gtgttccagt agctaccgtc gctattaata atagtacgaa
     4381 cgctgcgctg ttggctgtca gactgcttgg cgcttatgat tcaagttata caacgaaaat
     4441 ggaacagttt ttattaaagc aagaagaaga agttcttgtc aaagcacaaa agttagaaac
     4501 tgtcggttac gaagcttatc tagaaaacaa gtaatatata agtttattga tatacttgta
     4561 cagcaaataa ttataaaatg atatacctat tttttaggct ttgttatgat tacatcaaat
     4621 gtggacttca tacatagaaa tcaacgctta caggtgtcct tttttaagaa tttcatacat
     4681 aagatctact agaatcaatg ttaaacctgt tatcattaga acagccatct ccagaaggtt
     4741 ctatccaagt gaaaactttg gtcttcaagc caaagactgc taaaacacaa accccagtcc
     4801 caattattgt tgttgctgaa acttctactt ctactccggg tggtgttata gccaaagctt
     4861 cgggcactaa agagccaaga ttagcaaacg atgaattgac caaccaattc tttggttctc
     4921 ctgctaaaga gttttctatt gccagcttat caaacgaaca caaagacggt aaagtcaaga
     4981 tcattattga tgctgggatc aaagatgatg actcgactaa ttacgttatt tccaacactg
     5041 ttgctaagtt agttttaacc ggtaagcaat tattaggttt cttatccaac tacgaaccaa
     5101 ccatcgttga ttttactgct gaagctgcaa ccgctgcccc aactccagtt gttaagaaag
     5161 aacctgctgc atctggtaag cttgatgatg ccaaattaat tggtgttact gctgacaagg
     5221 aaaaagattt ctccaactgg tactcccaaa ttctaatgaa gggtgaaatg ttagactact
     5281 acgatgtctc tggttgttac attatgagac cagcttctta cacaatttgg gaaaagattc
     5341 aatcttggtt cgattcgaag attaaagaag atggtgttac taatgcttat ttcccaatgt
     5401 ttgtctcatc caaggtttta gaaaaggaaa aggaccacat cgatggtttc gctcctgaag
     5461 ttgcttgggt taccagagct ggtaaatctg aattagaaga accaattgct attagaccaa
     5521 cttctgaaac tgttatgggt accctgcagg ggccggcctt aattaaggcg cgcccttcct
     5581 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5641 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5701 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5761 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5821 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5881 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5941 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6001 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6061 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6121 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6181 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6241 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6301 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6361 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6421 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6481 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6721 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6781 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7141 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7381 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7441 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7501 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     7561 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7621 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     7681 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     7741 gggcgaaaaa ccgtctatca agggcgatgg cccactac
//
