Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V000908 | TFEB promoter-luciferase reporter | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
PGC-1α promoted htt turnover and the elimination of protein aggregates by activating transcription factor EB (TFEB), a master regulator of the autophagy-lysosome pathway.Placing PGC-1α upstream of TFEB. PGC-1α and TFEB thus hold great promise as therapies for HD and other neurodegenerative proteinopathies
- Vector Name:
- TFEB promoter-luciferase reporter
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7001 bp
- Type:
- Luciferase
- Replication origin:
- ori
- Copy Number:
- Low Copy
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- RVprimer3 (CTAGCAAAATAGGCTGTCCC)
- 3' Primer:
- EBV rev
- Growth Strain(s):
- DH5alpha
- Growth Temperature:
- 37℃
TFEB promoter-luciferase reporter vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Tsunemi T, Ashe TD, Morrison BE, Soriano KR, Au J, Roque RA, Lazarowski ER, Damian VA, Masliah E, La Spada AR. PGC-1α rescues Huntington's disease proteotoxicity by preventing oxidative stress and promoting TFEB function. Sci Transl Med. 2012 Jul 11;4(142):142ra97.
TFEB promoter-luciferase reporter vector Sequence
LOCUS 40924_48932 7001 bp DNA circular SYN 13-MAY-2021 DEFINITION Reporter for activity of TFEB gene promoter. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7001) AUTHORS Tsunemi T, Ashe TD, Morrison BE, Soriano KR, Au J, Roque RA, Lazarowski ER, Damian VA, Masliah E, La Spada AR TITLE PGC-1alpha rescues Huntington's disease proteotoxicity by preventing oxidative stress and promoting TFEB function. JOURNAL Sci Transl Med. 2012 Jul 11;4(142):142ra97. doi: 10.1126/scitranslmed.3003799. PUBMED 22786682 REFERENCE 2 (bases 1 to 7001) TITLE Direct Submission REFERENCE 3 (bases 1 to 7001) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl Med."; date: "2012-07-11"; pages: " 10.1126/scitranslmed.3003799" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..7001 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal 69..117 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 131..222 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" CDS 2500..4149 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal complement(4193..4314) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4562..4579) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4733..5321) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5495..6352) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6353..6457) /label=AmpR promoter rep_origin 6484..6939 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"