TFEB promoter-luciferase reporter vector (V000908)

Price Information

Cat No. Plasmid Name Availability Add to cart
V000908 TFEB promoter-luciferase reporter In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

PGC-1α promoted htt turnover and the elimination of protein aggregates by activating transcription factor EB (TFEB), a master regulator of the autophagy-lysosome pathway.Placing PGC-1α upstream of TFEB. PGC-1α and TFEB thus hold great promise as therapies for HD and other neurodegenerative proteinopathies

      • Vector Name:
      • TFEB promoter-luciferase reporter
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7001 bp
      • Type:
      • Luciferase
      • Copy Number:
      • Low Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • RVprimer3 (CTAGCAAAATAGGCTGTCCC)
      • 3' Primer:
      • EBV rev
      • Growth Strain(s):
      • DH5alpha
      • Growth Temperature:
      • 37℃

TFEB promoter-luciferase reporter vector Vector Map

TFEB promoter-luciferase reporter7001 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900synthetic polyadenylation signalpause siteKozak sequenceSV40 poly(A) signalL4440oriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Tsunemi T, Ashe TD, Morrison BE, Soriano KR, Au J, Roque RA, Lazarowski ER, Damian VA, Masliah E, La Spada AR. PGC-1α rescues Huntington's disease proteotoxicity by preventing oxidative stress and promoting TFEB function. Sci Transl Med. 2012 Jul 11;4(142):142ra97.

TFEB promoter-luciferase reporter vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7001 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Reporter for activity of TFEB gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    TFEB promoter-luciferase reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Tsunemi T, Ashe TD, Morrison BE, Soriano KR, Au J, Roque RA, 
            Lazarowski ER, Damian VA, Masliah E, La Spada AR
  TITLE     PGC-1alpha rescues Huntington's disease proteotoxicity by preventing
            oxidative stress and promoting TFEB function.
  JOURNAL   Sci Transl Med. 2012 Jul 11;4(142):142ra97. doi: 
            10.1126/scitranslmed.3003799.
  PUBMED    22786682
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2012-07-11"; volume: "4(142):142ra97. doi"; pages: " 
            10.1126/scitranslmed.3003799"
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    69..117
                     /note="synthetic polyadenylation signal"
     misc_feature    131..222
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     171..190
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2494..2503
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2500..4152
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2566..2584)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4193..4314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4204..4223)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4258..4277
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4562..4579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4733..5321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4813..4832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5492..6352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6115..6134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6353..6457)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6484..6939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6571..6590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6781..6802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"