LOCUS       40924_48932        7001 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Reporter for activity of TFEB gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Tsunemi T, Ashe TD, Morrison BE, Soriano KR, Au J, Roque RA, 
            Lazarowski ER, Damian VA, Masliah E, La Spada AR
  TITLE     PGC-1alpha rescues Huntington's disease proteotoxicity by preventing
            oxidative stress and promoting TFEB function.
  JOURNAL   Sci Transl Med. 2012 Jul 11;4(142):142ra97. doi: 
            10.1126/scitranslmed.3003799.
  PUBMED    22786682
REFERENCE   2  (bases 1 to 7001)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl
            Med."; date: "2012-07-11"; pages: "
            10.1126/scitranslmed.3003799"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    69..117
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    131..222
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     CDS             2500..4149
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(4193..4314)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4562..4579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4733..5321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5495..6352)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6353..6457)
                     /label=AmpR promoter
     rep_origin      6484..6939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga
       61 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
      121 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
      181 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct
      241 cttacgcgtg ctagtgtggc cagatagggt gtggtgtgga gacagggaca tcactctgta
      301 ggagctagag aaggggtcgt gggaggtggt ggaggctgca gctggtagct gctacaggat
      361 gggggaaggg taggttgtca ttgcctgggt tgttgggggc ttccctgtag agcatgggct
      421 gctcaggccc cttcactggg cagagcatga gagaagatgc agtgctggcc tctgcatgca
      481 gcaggacaca caggacagtg ggtagtctca ctctattaca gagctgctta gatagtggct
      541 ggagtcttga ttgcagatat gggacctagg ctccactcct ggttctggat caaccagttt
      601 cccaactggg aacaactctg ccccccaggc ttccccttcc cagcatgggg tagaccagat
      661 gtcccagccc ccaggcctcc cttccccagc atggggtaga ccagatacct ggggtggggt
      721 cctagctttg tcctgcactg tgcctctgat ttcccttctt tatgaaagac tgtggtttgt
      781 aattaagcaa gggcctccaa acagggctga tggaggcctt ggatgccggg tttgaagacg
      841 ctagctagat tgtaccagtc tcatgtactc agactgccag gcactgcaca aagctgccag
      901 tttttactaa atgcagagga agataaactg aggctccagg attatagctt gaccaaggtc
      961 acataacaaa gaatcagcga gcgctctgct ccaccagccc tttggggaag ccgttgctca
     1021 atcatgaagt catgccttgc caaatccctc tagtccctaa ctgtactgtc atggtctcag
     1081 atacggccag accagtgcct atcagtcttg ggctgtccct gagaagtcac tgggttctgc
     1141 tctttccttt gtggcctaga gaggggaagg gacttggtta ctgtcactca ttgagctaga
     1201 gacagagcga gcatgccgag ttgcctgccc cccttcagcc ttggttgggg cctcagccac
     1261 accttcccac agtgggtcaa gctctgagct ccgagcagtg aggagccctg aggtggtgat
     1321 tctctgcctg tgggtcttcc tttctctcgc cgactttggg cgccaggagc cagggacggg
     1381 gtacatctcc agtgcctggt tcatggataa tatccattct catttgcttc atgcaatggc
     1441 ttctttctgt ctcccatgac ccaacggcta gaggccgaag gtctagcagt aaacaaacag
     1501 atgaaatgtg tcccctcagg ggacttgcat tcctagtaag ggacagacat ggaataacaa
     1561 taacaaacgt aataagaagc aaaaaaaaaa aatatgttct gtcctagatg gtggcatctg
     1621 atatagagaa gcaaggtggt ggtggtatgc cattttagca cagtggtcct gaaatggcac
     1681 tctgaggtga cattagagtc tctcggagga ggtgaagaag tgagccttga tggtatctgt
     1741 gtaaagaaca ttccaggtgg aggcaacagc aagtgcaaag gtgggggggg gggacggtgg
     1801 ggggcggaga aagcatgcct ggagtgtttg aaggatagtt taaggctggg ggtgtggctg
     1861 gagcagggag aacaagggag agaagcatgt gttgggggcg ggaagtctat gggggaccat
     1921 gagggtgcag cagattacag aggaccattt aggctgggtt taaggattta ggcttttgtt
     1981 ccgatgaact gggagctatg gagagttggg agcagacagc agggcaggaa ctaacaggat
     2041 gatggagcta ctgtgagcag atggtggggg aggagcacgg aggagcatct ggaggctcct
     2101 caggccctga ggaggcgtgg tgcaggctgg cctggggagg ggcgggagag ggggccggag
     2161 ctgaggccag gtatgaaata ctcaggatgt ggatgtgaca gcgaggggtc aggtggactc
     2221 ctgcagtcac tgaaggacag tgttcacctc tggtctagaa ctcagggaac ttgggttcat
     2281 ggctcagctc gctcagtggt cttgggcaaa tcccttctgc ccggactcag tttctccttg
     2341 tgcacaatgg gagcaaccat acttatgcca gcctgtgctt aaagactacg aagatgatga
     2401 atacttcatg ggcctgtctc ccctcgacta cagggagccc gaaccaacag ctgccatccc
     2461 ggggaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
     2521 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     2581 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     2641 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     2701 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     2761 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     2821 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     2881 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     2941 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     3001 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     3061 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     3121 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     3181 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     3241 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     3301 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     3361 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     3421 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     3481 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     3541 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     3601 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     3661 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     3721 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     3781 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     3841 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     3901 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     3961 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     4021 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     4081 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     4141 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     4201 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4261 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     4321 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     4381 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
     4441 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     4501 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     4561 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4621 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4681 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4741 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4801 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4861 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4921 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4981 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5041 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5101 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5161 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5221 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5341 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5401 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5461 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5521 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5581 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5641 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5701 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5761 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5821 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5881 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5941 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6001 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6061 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6121 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6181 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6241 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6301 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6361 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6421 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6481 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6541 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6601 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6661 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6721 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6781 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6841 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6901 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     6961 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct a
//
