Basic Vector Information
YIpMELalpha vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YIpMELalpha vector Sequence
LOCUS 40924_49767 6716 bp DNA circular SYN 18-DEC-2018 DEFINITION UAS-less reporter vector YIpMELalpha, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6716) AUTHORS Melcher K, Sharma B, Ding WV, Nolden M. TITLE Zero background yeast reporter plasmids JOURNAL Gene 247 (1-2), 53-61 (2000) PUBMED 10773444 REFERENCE 2 (bases 1 to 6716) AUTHORS Melcher K, Sharma B, Ding WV, Nolden M. TITLE Direct Submission JOURNAL Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt University, Marie Curie Strasse 9, Frankfurt 60439, Germany REFERENCE 3 (bases 1 to 6716) TITLE Direct Submission REFERENCE 4 (bases 1 to 6716) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247 (1-2), 53-61 (2000)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt University, Marie Curie Strasse 9, Frankfurt 60439, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6716 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(413..1825) /gene="MEL1" /label=MEL1 /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae. Accession#: P04824" regulatory complement(1826..2478) /label=UAS deleted MEL1 promoter /note="UAS deleted MEL1 promoter" /regulatory_class="promoter" primer_bind complement(2488..2504) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(2780..3580) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(3581..3791) /label=URA3 promoter promoter 4595..4699 /label=AmpR promoter CDS 4700..5557 /label=AmpR /note="beta-lactamase" rep_origin 5731..6319 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 6607..6628 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 6643..6673 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6681..6697 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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