LOCUS       V001474                 6716 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001474
VERSION     V001474
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
   PUBMED   10773444
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 6716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247
            (1-2), 53-61 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-SEP-2003) Institute for Microbiology, Frankfurt University,
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(413..1825)
                     /gene="MEL1"
                     /label="Alpha-galactosidase 1"
                     /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae.
                     Accession#: P04824"
     regulatory      complement(1826..2478)
                     /label="UAS deleted MEL1 promoter"
                     /note="UAS deleted MEL1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2488..2504)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2780..3580)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3581..3791)
                     /label="URA3 promoter"
     promoter        4595..4699
                     /label="AmpR promoter"
     CDS             4700..5557
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5731..6319
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6607..6628
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6643..6673
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6681..6697
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgacatgat tacgaattcg agctcggtac ctgtagacta gcgccttctg cttcttcgat
       61 atccggtttt gctgatatga tgatttcgtc gtttgtatgt actcctgaag ctgttgttcc
      121 tcttcgtgaa atgtcataca attctagaat cacattggca gaaaagtatt ttacaaaagt
      181 cactagtata gcacatgtga aatatcgcta atgcaatgaa gtatgtaaac gagaaaaagt
      241 tcaagggctt cagattgtgt acctacaaaa cgaaaactat tggaatacct gataggctat
      301 tctgtcgttt attatttagt tactctgaat aaatattcga gtagtatttt tttcatgaac
      361 tttttaacaa tcatcaatac ttctcgccct gctttgctca acaataagct caagaagagg
      421 gtctcaacct atagaaggcg ataccgtgag ctggaacagt cgtgttaagt atagcatttg
      481 gagaaagact accaattttc tgaccaaaca gtcttgtatc attcttagac aaaccgtctt
      541 tgtaggattg ctccgtagca ttgtagagaa taccggtggc tgtcttattc cgtccaagga
      601 tagcagacgc tgtcgagttg tcaactctgt tggcccatag gtcgtagata tcccaagtcg
      661 atgtcagttt ctttgaaccc agattgctgt caaaaaaaat ctcttccaag gtcgtgttca
      721 ttggtctaga tacgcttcct ccattcaata aagcaaccac ttgatcacca ttgtcaagag
      781 gaccactcca catttgaatt tcaccttgtc catattcatc tgtgtctgaa acataatatc
      841 tccagactct tgttgctgga ataccatttg aatcttgatt aattgcgatg acagaggctt
      901 gactatagat tgagtacgaa gatgccttta agttattcac attggcacca atgataagtg
      961 gagactttac cattgcccac atagagaaat gtgccttttc ctcatcgtca gtcaaattcc
     1021 cgacaccaac ctctagattg tccagatcat tccaaccacc aacacctgca ttttgcccca
     1081 ttggagctgc cttgttaaga atattcataa tagaacaatg gaaaccggcg tacttgcaat
     1141 cgtattcatc gccatcacag ggacatctgc tatctggacg agtgaactca gcagtaacat
     1201 ctccactcat tctccaagaa ttggcgatac cagagcccca gtaaaatgtt aaatcctgac
     1261 cccagttaca tagagaatag aatataggcc taccagtttt attcaaagca tctgacatgg
     1321 ccttgtaacg gtggtaagaa atttccggtg taccaaactg acccttattg taacaattat
     1381 cgtacttcaa gtagtcaacg cggttatttg caaagaactg tgcatcttct tcctcacgac
     1441 ccagagaccc aggatatcca gcacaggtgt actcaccagc agacgaatac ataccgaaaa
     1501 gaaagctgtt attatgcagg tggtctgcaa catggcccat accattggga aatttttgtt
     1561 catctgcaac gaggaaaccg tcggaatctc tgccgctaga ccagcagtca tccagaatga
     1621 tatacttgta acccatatcc tttagcccca agtcagaaat tctatcagcg gtgtctagaa
     1681 gtagctgttc actgacatcg caggcaaacg tattccagtt gtcccaaccc atctgtggag
     1741 tgagaccaag gccattgtaa ctcggagaca ccccaaaaac gcccttcaaa ctgatgcatg
     1801 cggtgagaaa gtagaaagca aacatcgtcg ttgcttttat taccgttgct ctcctagaat
     1861 caagtaagaa attatcgaaa accttataca gtaagaagtt tatgttttat tgaggactta
     1921 agtacagttt ttatagtttt tatagttttc ataattttta ttcagctact tcgttcaatg
     1981 tctttaagat tcttgatttg tagacgtcaa gaaccatttt ttgttggata atgtaataca
     2041 tgtcttcctt tcttcttctc gagtgaaagc caatgcgtcc tgactattcc ctggaaaaac
     2101 ctttcaggga tgggatgtct ttctcccccc acattaatgt cgattaaaca cgagaatgca
     2161 ttaaaacaaa catgttatgt ttatggcaga gactagacgc attcagacca ccatgtgttt
     2221 tccagattta gtcatctcct agtaatggtg tttacttaga agtcgacttc ggtagggtgc
     2281 tatcctttaa aggcccaccc tgagcgtaca gaaagaattc taggggaaga cggggtagaa
     2341 acttagtgtt gatgtctcct gaagacttgt tgnctttggc aatagcagca gcaaaaaaaa
     2401 atgggggttt tacacaaatt tccaaagtct tatcatcacg tgcatttttc agtatacata
     2461 atattaaagt aaaaaagaaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     2521 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg ccagctggcg
     2581 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2641 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg
     2701 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
     2761 cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg
     2821 cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc
     2881 caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac
     2941 ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac
     3001 cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc
     3061 tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg
     3121 acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct
     3181 tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt
     3241 ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa
     3301 attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa
     3361 ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt
     3421 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
     3481 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
     3541 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcggtt
     3601 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     3661 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     3721 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     3781 gatgaattga aaagctcttg ttacccatca ttgaattttg aacatccgaa cctgggagtt
     3841 ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg
     3901 gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc
     3961 ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc
     4021 tttgttattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     4081 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     4141 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     4201 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     4261 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgttatggtg
     4321 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4381 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4441 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4501 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4561 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4921 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5221 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6601 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagct
//
