YIpDCE1 vector (V001475)

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LOCUS       40924_49747        8655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrating expression vector YIpDCE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Stotz A, Linder P.
  TITLE     The ADE2 gene from Saccharomyces cerevisiae: sequence and new 
            vectors
  JOURNAL   Gene 95 (1), 91-98 (1990)
  PUBMED    2253890
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     YIpDCE1 - an integrating plasmid for dual constitutive expression in
            yeast
  JOURNAL   Gene 212 (2), 197-202 (1998)
  PUBMED    9611262
REFERENCE   3  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, 
            Bethesda, MD 20892, USA
REFERENCE   4  (bases 1 to 8655)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1990"; volume: "95"; issue: "1"; pages: "91-98"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "212"; issue: "2"; pages: "197-202"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, Bethesda, MD 
            20892, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      306..1800
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="promoter"
     misc_feature    1801..1846
                     /note="polylinker; multiple cloning site 1"
     terminator      1943..2216
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     primer_bind     complement(2263..2279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2287..2303)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2311..2341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2356..2377)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2442..2817
                     /label=ADE2 promoter
     CDS             2818..4530
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
     regulatory      4808..6300
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="promoter"
     misc_feature    6301..6330
                     /note="polylinker; multiple cloning site 2"
     regulatory      6331..6707
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="terminator"
     rep_origin      complement(6836..7424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7598..8455)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8456..8560)
                     /label=AmpR promoter