Basic Vector Information
YIpDCE1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YIpDCE1 vector Sequence
LOCUS 40924_49747 8655 bp DNA circular SYN 18-DEC-2018 DEFINITION Integrating expression vector YIpDCE1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8655) AUTHORS Stotz A, Linder P. TITLE The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors JOURNAL Gene 95 (1), 91-98 (1990) PUBMED 2253890 REFERENCE 2 (bases 1 to 8655) AUTHORS Stearman R, Dancis A, Klausner RD. TITLE YIpDCE1 - an integrating plasmid for dual constitutive expression in yeast JOURNAL Gene 212 (2), 197-202 (1998) PUBMED 9611262 REFERENCE 3 (bases 1 to 8655) AUTHORS Stearman R, Dancis A, Klausner RD. TITLE Direct Submission JOURNAL Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, Bethesda, MD 20892, USA REFERENCE 4 (bases 1 to 8655) TITLE Direct Submission REFERENCE 5 (bases 1 to 8655) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1990"; volume: "95"; issue: "1"; pages: "91-98" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; date: "1998"; volume: "212"; issue: "2"; pages: "197-202" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, Bethesda, MD 20892, USA" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..8655 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 306..1800 /label=phosphoglycerate kinase /note="phosphoglycerate kinase" /regulatory_class="promoter" misc_feature 1801..1846 /note="polylinker; multiple cloning site 1" terminator 1943..2216 /label=PGK1 terminator /note="transcription terminator for 3-phosphoglycerate kinase gene" primer_bind complement(2263..2279) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2287..2303) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2311..2341) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2356..2377) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2442..2817 /label=ADE2 promoter CDS 2818..4530 /label=ADE2 /note="phosphoribosylaminoimidazole carboxylase, required for adenine biosynthesis" regulatory 4808..6300 /label=phosphoglycerate kinase /note="phosphoglycerate kinase" /regulatory_class="promoter" misc_feature 6301..6330 /note="polylinker; multiple cloning site 2" regulatory 6331..6707 /label=phosphoglycerate kinase /note="phosphoglycerate kinase" /regulatory_class="terminator" rep_origin complement(6836..7424) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7598..8455) /label=AmpR /note="beta-lactamase" promoter complement(8456..8560) /label=AmpR promoter
This page is informational only.