YIpDCE1 vector (V001475)

Basic Vector Information

      • Vector Name:
      • YIpDCE1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8655 bp
      • Type:
      • Integrating expression vector
      • Source/Author:
      • Stotz A, Linder P.

YIpDCE1 vector Vector Map

YIpDCE18655 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400phosphoglycerate kinasepolylinker; multiple cloning site 1phosphoglycerate kinaseM13 revlac operatorlac promoterCAP binding siteADE2 promoterADE2phosphoglycerate kinasepolylinker; multiple cloning site 2phosphoglycerate kinaseoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YIpDCE1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8655 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integrating expression vector YIpDCE1, complete sequence.
ACCESSION   AF039102
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Stotz A, Linder P.
  TITLE     The ADE2 gene from Saccharomyces cerevisiae: sequence and new 
            vectors
  JOURNAL   Gene 95 (1), 91-98 (1990)
  PUBMED    2253890
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     YIpDCE1 - an integrating plasmid for dual constitutive expression in
            yeast
  JOURNAL   Gene 212 (2), 197-202 (1998)
  PUBMED    9611262
REFERENCE   3  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, 
            Bethesda, MD 20892, USA
REFERENCE   4  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Integrating expression vector YIpDCE1"
                     /host="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:75737"
     regulatory      306..1800
                     /regulatory_class="promoter"
                     /note="phosphoglycerate kinase"
     misc_feature    1801..1846
                     /note="polylinker; multiple cloning site 1"
     regulatory      1847..2223
                     /regulatory_class="terminator"
                     /note="phosphoglycerate kinase"
     primer_bind     complement(2263..2279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2287..2303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2311..2341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2356..2377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2442..2817
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             2818..4533
                     /codon_start=1
                     /gene="ADE2"
                     /product="phosphoribosylamino-imidazole-carboxylase"
                     /function="adenine biosynthesis"
                     /label=ADE2
                     /protein_id="AAC40224.1"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     gene            2818..4533
                     /gene="ADE2"
                     /label=ADE2
     regulatory      4808..6300
                     /regulatory_class="promoter"
                     /note="phosphoglycerate kinase"
     misc_feature    6301..6330
                     /note="polylinker; multiple cloning site 2"
     regulatory      6331..6707
                     /regulatory_class="terminator"
                     /note="phosphoglycerate kinase"
     rep_origin      complement(6836..7424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7595..8455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8456..8560)
                     /gene="bla"
                     /label=AmpR promoter

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