LOCUS       40924_49747        8655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrating expression vector YIpDCE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Stotz A, Linder P.
  TITLE     The ADE2 gene from Saccharomyces cerevisiae: sequence and new 
            vectors
  JOURNAL   Gene 95 (1), 91-98 (1990)
  PUBMED    2253890
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     YIpDCE1 - an integrating plasmid for dual constitutive expression in
            yeast
  JOURNAL   Gene 212 (2), 197-202 (1998)
  PUBMED    9611262
REFERENCE   3  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, 
            Bethesda, MD 20892, USA
REFERENCE   4  (bases 1 to 8655)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1990"; volume: "95"; issue: "1"; pages: "91-98"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "212"; issue: "2"; pages: "197-202"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, Bethesda, MD 
            20892, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      306..1800
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="promoter"
     misc_feature    1801..1846
                     /note="polylinker; multiple cloning site 1"
     terminator      1943..2216
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     primer_bind     complement(2263..2279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2287..2303)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2311..2341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2356..2377)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2442..2817
                     /label=ADE2 promoter
     CDS             2818..4530
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
     regulatory      4808..6300
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="promoter"
     misc_feature    6301..6330
                     /note="polylinker; multiple cloning site 2"
     regulatory      6331..6707
                     /label=phosphoglycerate kinase
                     /note="phosphoglycerate kinase"
                     /regulatory_class="terminator"
     rep_origin      complement(6836..7424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7598..8455)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8456..8560)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagag ctttctaact gatctatcca aaactgaaaa ttacattctt gattaggttt
      361 atcacaggca aatgtaattt gtggtatttt gccgttcaaa atctgtagaa ttttctcatt
      421 ggtcacatta caacctgaaa atactttatc tacaatcata ccattcttat aacatgtccc
      481 cttaatacta ggatcaggca tgaacgcatc acagacaaaa tcttcttgac aaacgtcaca
      541 attgatccct ccccatccgt tatcacaatg acaggtgtca ttttgtgctc ttatgggacg
      601 atccttatta ccgctttcat ccggtgatag accgccacag aggggcagag agcaatcatc
      661 acctgcaaac ccttctatac actcacatct accagtgtac gaattgcatt cagaaaactg
      721 tttgcattca aaaataggta gcatacaatt aaaacatggc gggcacgtat cattgccctt
      781 atcttgtgca gttagacgcg aatttttcga agaagtacct tcaaagaatg gggtctcatc
      841 ttgttttgca agtaccactg agcaggataa taatagaaat gataatatac tatagtagag
      901 ataacgtcga tgacttccca tactgtaatt gcttttagtt gtgtattttt agtgtgcaag
      961 tttctgtaaa tcgattaatt tttttttctt tcctcttttt attaacctta atttttattt
     1021 tagattcctg acttcaactc aagacgcaca gatattataa catctgcaca ataggcattt
     1081 gcaagaatta ctcgtgagta aggaaagagt gaggaactat cgcatacctg catttaaaga
     1141 tgccgatttg ggcgcgaatc ctttattttg gcttcaccct catactatta tcagggccag
     1201 aaaaaggaag tgtttccctc cttcttgaat tgatgttacc ctcataaagc acgtggcctc
     1261 ttatcgagaa agaaattacc gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa
     1321 aacccagaca cgctcgactt cctgtcttcc tattgattgc agcttccaat ttcgtcacac
     1381 aacaaggtcc tagcgacggc tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg
     1441 cgggaaaggg tttagtacca catgctatga tgcccactgt gatctccaga gcaaagttcg
     1501 ttcgatcgta ctgttactct ctctctttca aacagaattg tccgaatcgt gtgacaacaa
     1561 cagcctgttc tcacacactc ttttcttcta accaaggggg tggtttagtt tagtagaacc
     1621 tcgtgaaact tacatttaca tatatataaa cttgcataaa ttggtcaatg caagaaatac
     1681 atatttggtc ttttctaatt cgtagttttt caagttctta gatgctttct ttttctcttt
     1741 tttacagatc atcaaggaag taattatcta ctttttacaa caaatataaa accaaaagat
     1801 ctgagctcgt cgacgtatac ggatccatga aaccatggag ttgaattcga tctcccatgt
     1861 ctctactggt ggtggtgctt ctttggaatt attggaaggt aaggaattgc caggtgttgc
     1921 tttcttatcc gaaaagaaat aaattgaatt gaattgaaat cgatagatca atttttttct
     1981 tttctctttc cccatccttt acgctaaaat aatagtttat tttatttttt gaatattttt
     2041 tatttatata cgtatatata gactattatt tacttttaat agattattaa gatttttatt
     2101 aaaaaaaaat tcgtccctct ttttaatgcc ttttatgcag tttttttttc ccattcgata
     2161 tttctatgtt cgggtttcag cgtattttaa gtttaataac tcgaaaattc tgcgtttcga
     2221 aaaagcttcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     2281 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2341 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2401 tttccagtcg ggaaacctgt cgtgccagca gatctgaatt attcttgaat aatacataac
     2461 ttttcttaaa agaatcaaag acagataaaa tttaagagat attaaatatt agtgagaagc
     2521 cgagaatttt gtaacaccaa cataacactg acatctttaa caacttttaa ttatgataca
     2581 tttcttacgt catgattgat tattacagct atgctgacaa atgactcttg ttgcatggct
     2641 acgaaccggg taatactaag tgattgactc ttgctgacct tttattaaga actaaatgga
     2701 caatattatg gagcatttca tgtataaatt ggtgcgtaaa atcgttggat ctctcttcta
     2761 agtacatcct actataacaa tcaagaaaaa caagaaaatc ggacaaaaca atcaagtatg
     2821 gattctagaa cagttggtat attaggaggg ggacaattgg gacgtatgat tgttgaggca
     2881 gcaaacaggc tcaacattaa gacggtaata ctagatgctg aaaattctcc tgccaaacaa
     2941 ataagcaact ccaatgacca cgttaatggc tccttttcca atcctcttga tatcgaaaaa
     3001 ctagctgaaa aatgtgatgt gctaacgatt gagattgagc atgttgatgt tcctacacta
     3061 aagaatcttc aagtaaaaca tcccaaatta aaaatttacc cttctccaga aacaatcaga
     3121 ttgatacaag acaaatatat tcaaaaagag catttaatca aaaatggtat agcagttacc
     3181 caaagtgttc ctgtggaaca agccagtgag acgtccctat tgaatgttgg aagagatttg
     3241 ggttttccat tcgtcttgaa gtcgaggact ttggcatacg atggaagagg taacttcgtt
     3301 gtaaagaata aggaaatgat tccggaagct ttggaagtac tgaaggatcg tcctttgtac
     3361 gccgaaaaat gggcaccatt tactaaagaa ttagcagtca tgattgtgag gtctgttaac
     3421 ggtttagtgt tttcttaccc aattgtagag actatccaca aggacaatat ttgtgactta
     3481 tgttatgcgc ctgctagagt tccggactcc gttcaactta aggcgaagtt gttggcagaa
     3541 aatgcaatca aatcttttcc cggttgtggt atatttggtg tggaaatgtt ctatttagaa
     3601 acaggggaat tgcttattaa cgaaattgcc ccaaggcctc acaactctgg acattatacc
     3661 attgatgctt gcgtcacttc tcaatttgaa gctcatttga gatcaatatt ggatttgcca
     3721 atgccaaaga atttcacatc tttctccacc attacaacga acgccattat gctaaatgtt
     3781 cttggagaca aacatacaaa agataaagag ctagaaactt gcgaaagagc attggcgact
     3841 ccaggttcct cagtgtactt atatggaaaa gagtctagac ctaacagaaa agtaggtcac
     3901 ataaatatta ttgcctccag tatggcggaa tgtgaacaaa ggctgaacta cattacaggt
     3961 agaactgata ttccaatcaa aatctctgtc gctcaaaagt tggacttgga agcaatggtc
     4021 aaaccattgg ttggaatcat catgggatca gactctgact tgccggtaat gtctgccgca
     4081 tgtgcggttt taaaagattt tggcgttcca tttgaagtga caatagtctc tgctcataga
     4141 actccacata ggatgtcagc atatgctatt tccgcaagca agcgtggaat taaaacaatt
     4201 atcgctggag ctggtggggc tgctcacttg ccaggtatgg tggctgcaat gacaccactt
     4261 cctgtcatcg gtgtgcccgt aaaaggttct tgtctagatg gagtagattc tttacattca
     4321 attgtgcaaa tgcctagagg tgttccagta gctaccgtcg ctattaataa tagtacgaac
     4381 gctgcgctgt tggctgtcag actgcttggc gcttatgatt caagttatac aacgaaaatg
     4441 gaacagtttt tattaaagca agaagaagaa gttcttgtca aagcacaaaa gttagaaact
     4501 gtcggttacg aagcttatct agaaaacaag taatatataa gtttattgat atacttgtac
     4561 agcaaataat tataaaatga tatacctatt ttttaggctt tgttatgatt acatcaaatg
     4621 tggacttcat acatagaaat caacgcttac aggtgtcctt ttttaagaat ttcatacata
     4681 agatctgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4801 atcagcatcg agctttctaa ctgatctatc caaaactgaa aattacattc ttgattaggt
     4861 ttatcacagg caaatgtaat ttgtggtatt ttgccgttca aaatctgtag aattttctca
     4921 ttggtcacat tacaacctga aaatacttta tctacaatca taccattctt ataacatgtc
     4981 cccttaatac taggatcagg catgaacgca tcacagacaa aatcttcttg acaaacgtca
     5041 caattgatcc ctccccatcc gttatcacaa tgacaggtgt cattttgtgc tcttatggga
     5101 cgatccttat taccgctttc atccggtgat agaccgccac agaggggcag agagcaatca
     5161 tcacctgcaa acccttctat acactcacat ctaccagtgt acgaattgca ttcagaaaac
     5221 tgtttgcatt caaaaatagg tagcatacaa ttaaaacatg gcgggcacgt atcattgccc
     5281 ttatcttgtg cagttagacg cgaatttttc gaagaagtac cttcaaagaa tggggtctca
     5341 tcttgttttg caagtaccac tgagcaggat aataatagaa atgataatat actatagtag
     5401 agataacgtc gatgacttcc catactgtaa ttgcttttag ttgtgtattt ttagtgtgca
     5461 agtttctgta aatcgattaa tttttttttc tttcctcttt ttattaacct taatttttat
     5521 tttagattcc tgacttcaac tcaagacgca cagatattat aacatctgca caataggcat
     5581 ttgcaagaat tactcgtgag taaggaaaga gtgaggaact atcgcatacc tgcatttaaa
     5641 gatgccgatt tgggcgcgaa tcctttattt tggcttcacc ctcatactat tatcagggcc
     5701 agaaaaagga agtgtttccc tccttcttga attgatgtta ccctcataaa gcacgtggcc
     5761 tcttatcgag aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa
     5821 aaaacccaga cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac
     5881 acaacaaggt cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat
     5941 ggcgggaaag ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt
     6001 cgttcgatcg tactgttact ctctctcttt caaacagaat tgtccgaatc gtgtgacaac
     6061 aacagcctgt tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa
     6121 cctcgtgaaa cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat
     6181 acatatttgg tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct
     6241 tttttacaga tcatcaagga agtaattatc tactttttac aacaaatata aaaccaaaag
     6301 atctggtacc ctcgagacta gtcctagggc ccgatctccc atgtctctac tggtggtggt
     6361 gcttctttgg aattattgga aggtaaggaa ttgccaggtg ttgctttctt atccgaaaag
     6421 aaataaattg aattgaattg aaatcgatag atcaattttt ttcttttctc tttccccatc
     6481 ctttacgcta aaataatagt ttattttatt ttttgaatat tttttattta tatacgtata
     6541 tatagactat tatttacttt taatagatta ttaagatttt tattaaaaaa aaattcgtcc
     6601 ctctttttaa tgccttttat gcagtttttt tttcccattc gatatttcta tgttcgggtt
     6661 tcagcgtatt ttaagtttaa taactcgaaa attctgcgtt tcgaaaaagc tcgatgctca
     6721 ctcaaaggtc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7261 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7441 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7501 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7561 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7621 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7681 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7741 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7801 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7861 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8581 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8641 cgaggccctt tcgtc
//
