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YDp-W vector (V001495#)

Basic Vector Information

Basic Vector Information
Vector Name YDp-W Antibiotic Resistance Ampicillin
Length 3536 bp Type Cloning vector
Source Stolz J.

YDp-W vector Vector Map

TRP1 promoter TRP1 M13 rev lac operator lac promoter CAP binding site ori AmpR AmpR promoter M13 fwd

YDp-W vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                3536 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-W, complete sequence.
ACCESSION   AY157312
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Cloning vector YDp-W"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:209527"
     promoter        37..138
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             139..813
                     /codon_start=1
                     /gene="TRP1"
                     /product="phosphoribosylanthranilate isomerase"
                     /EC_number="5.3.1.24"
                     /label=TRP1
                     /note="derived from Saccharomyces cerevisiae TRP1 gene; 
                     N-(5'-phosphoribosyl)anthranilate isomerase; Trp1p"
                     /protein_id="AAN31953.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     gene            139..813
                     /gene="TRP1"
                     /label=TRP1
     primer_bind     complement(920..936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    944..960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(968..998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1013..1034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2081..2941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2942..3046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3520..3536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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