YDp-W vector (V001495)

Basic Vector Information

      • Vector Name:
      • YDp-W
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3536 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Stolz J.

YDp-W vector Vector Map

YDp-W3536 bp6001200180024003000TRP1 promoterTRP1M13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YDp-W vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3536 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-W, complete sequence.
ACCESSION   AY157312
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Cloning vector YDp-W"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:209527"
     promoter        37..138
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             139..813
                     /codon_start=1
                     /gene="TRP1"
                     /product="phosphoribosylanthranilate isomerase"
                     /EC_number="5.3.1.24"
                     /label=TRP1
                     /note="derived from Saccharomyces cerevisiae TRP1 gene; 
                     N-(5'-phosphoribosyl)anthranilate isomerase; Trp1p"
                     /protein_id="AAN31953.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     gene            139..813
                     /gene="TRP1"
                     /label=TRP1
     primer_bind     complement(920..936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    944..960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(968..998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1013..1034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2081..2941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2942..3046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3520..3536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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